SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW~74 kDa)
JNK2 recombinant protein
JNK2, Unactive recombinant protein
Gene Names
Mapk9; SAPK
Applications
Western Blot
Purity
>90%
Synonyms
JNK2; N/A; JNK2, Unactive recombinant protein; SAPK; MAPK9; JNK2 recombinant protein
Host
E Coli
Purity/Purification
>90%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Concentration
0.2mg/ml (varies by lot)
Sequence Length
3711
Applicable Applications for JNK2 recombinant protein
WB (Western Blot), Kinase Assay
Type
Recombinant Fusion Protein
Species
Rat
Tag Information
GST tag
Expression System
E.coli
Source Note
Recombinant full-length rat JNK2 expressed in E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW~74 kDa)
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx. MW~74 kDa)
Related Product Information for JNK2 recombinant protein
Recombinant full-length rat JNK2 was expressed in E. coli cells using an N-terminal GST tag.
Scientific Background: JNK2 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation and treatment of cells with TNFα. JNK2 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK2 has been shown to play an important role in disease processes. JNK2 plays a role in ventricular hypertrophy and is thought to be involved in hypertensive cardiac disease (2).
Scientific Background: JNK2 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation and treatment of cells with TNFα. JNK2 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK2 has been shown to play an important role in disease processes. JNK2 plays a role in ventricular hypertrophy and is thought to be involved in hypertensive cardiac disease (2).
Product Categories/Family for JNK2 recombinant protein
References
1. Sluss, H K. et al: Signal transduction by tumor necrosis factor mediated by JNK protein kinases. Mol Cell Biol. 1994 Dec;14(12):8376-84.
2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.
2. Vogel, V. et al: Cardiac hypertrophy in the Prague-hypertensive rat is associated with enhanced JNK2 but not ERK tissue activity. Kidney Blood Press Res. 2001;24(1):52-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~74 kDa
NCBI Official Full Name
Rattus norvegicus mitogen-activated protein kinase 9 (Mapk9), transcript variant 1, mRNA
NCBI Official Synonym Full Names
mitogen-activated protein kinase 9
NCBI Official Symbol
Mapk9
NCBI Official Synonym Symbols
SAPK
NCBI Protein Information
mitogen-activated protein kinase 9; MAPK 9; p54-alpha; SAPK-alpha; MAP kinase 9; c-Jun N-terminal kinase 2; stress-activated protein kinase JNK2; stress activated protein kinase alpha II
UniProt Protein Name
Mitogen-activated protein kinase 9
UniProt Gene Name
Mapk9
UniProt Synonym Gene Names
Jnk2; Prkm9; MAP kinase 9; MAPK 9
UniProt Entry Name
MK09_RAT
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Product Notes
The JNK2 mapk9 (Catalog #AAA72883) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||E.coli!!Source Note||Recombinant full-length rat JNK2 expressed in E. coli cells. AAA Biotech's JNK2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the JNK2 mapk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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