Application Data
(HTRF assay for MBD2-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD2-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MBD2-MBD recombinant protein
Recombinant MBD2-MBD protein
Synonyms
MBD2-MBD; N/A; Recombinant MBD2-MBD protein; Methyl-CpG -binding domain protein 2; CXXC3; PCM1; Protein Containing Methyl-CpG Binding Domain 1; MBD2-MBD recombinant protein
Host
E coli
Form/Format
Recombinant MBD2-MBD protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant MBD2-MBD protein that includes amino acids 140-219 of human MBD2 protein (accession number NP_003918.1) was expressed in E coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 11.19kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(HTRF assay for MBD2-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD2-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for MBD2-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD2-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(MBD2-MBD protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 11.19kDa Purity: >95%)
SDS-PAGE
(MBD2-MBD protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 11.19kDa Purity: >95%)
Related Product Information for MBD2-MBD recombinant protein
MBD2 (Methyl-CpG-binding domain protein 2)is a transcriptional repressor that Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Also binds hemimethylated DNA as well and recruits histone deacetylases and DNA methyltransferases to chromatin. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Product Categories/Family for MBD2-MBD recombinant protein
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Product Notes
The MBD2-MBD (Catalog #AAA60371) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MBD2-MBD, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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