WB (Western Blot)
(MBD2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).)
Rabbit MBD2 Polyclonal Antibody | anti-MBD2 antibody
MBD2 Antibody (Center)
Gene Names
MBD2; DMTase; NY-CO-41
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MBD2, Antibody; MBD2 Antibody (Center); Methyl-CpG-binding domain protein 2; Demethylase; DMTase; Methyl-CpG-binding protein MBD2; MBD2; anti-MBD2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human MBD2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.29 (varies by lot)
Sequence Positions
250-279
Sequence Length
411
Applicable Applications for anti-MBD2 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(MBD2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).)
WB (Western Blot)
(MBD2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with MBD2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with MBD2 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.)
Related Product Information for anti-MBD2 antibody
DNA methylation is the major modification of eukaryotic
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. The protein encoded by this gene may function as a
mediator of the biological consequences of the methylation signal.
It is also reported that the this protein functions as a
demethylase to activate transcription, as DNA methylation causes
gene silencing.
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MECP2,
MBD1 and MBD2 can also repress transcription from methylated gene
promoters. The protein encoded by this gene may function as a
mediator of the biological consequences of the methylation signal.
It is also reported that the this protein functions as a
demethylase to activate transcription, as DNA methylation causes
gene silencing.
References
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
McDonough, C.W., et al. Hum. Genet. (2009) In press :
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010)
Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009)
McDonough, C.W., et al. Hum. Genet. (2009) In press :
Shen, M., et al. Environ. Mol. Mutagen. 50(4):285-290(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43255
NCBI Official Full Name
methyl-CpG-binding domain protein 2 isoform 1
NCBI Official Synonym Full Names
methyl-CpG binding domain protein 2
NCBI Official Symbol
MBD2
NCBI Official Synonym Symbols
DMTase; NY-CO-41
NCBI Protein Information
methyl-CpG-binding domain protein 2
UniProt Protein Name
Methyl-CpG-binding domain protein 2
UniProt Gene Name
MBD2
UniProt Synonym Gene Names
DMTase
UniProt Entry Name
MBD2_HUMAN
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Product Notes
The MBD2 mbd2 (Catalog #AAA283773) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 250-279. The MBD2 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MBD2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MBD2 mbd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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