Application Data
(MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
METTL1/WDR4 recombinant protein
Recombinant METTL1/WDR4 complex
Synonyms
METTL1/WDR4; N/A; Recombinant METTL1/WDR4 complex; WD Repeat-Containing Protein 4; Methyltransferase-Like Protein 1; METTL1/WDR4 recombinant protein
Host
E coli
Form/Format
Recombinant METTL1/WDR4 Complex is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant METTL1/WDR4 Complex that includes full length human WDR4 protein (accession number NP_061139.2) with an N-terminal T7 tag and C-terminal DYKDDDDK-His tag and full length human METTL1 protein (accession number NP_005362.3) was expressed in E coli. The molecular weights of WDR4 and METTL1 are 48.8kDa and 31.47kDa, respectively.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
Application Data
(MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
Application Data
(MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
Application Data
(MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
SDS-PAGE
(Recombinant METTL1/WDR4 protein gel. METTL1/WDR4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomasse Blue. MW: WDR4: 48.8kDa METTL1: 31.47kDa Purity: >90%)
SDS-PAGE
(Recombinant METTL1/WDR4 protein gel. METTL1/WDR4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomasse Blue. MW: WDR4: 48.8kDa METTL1: 31.47kDa Purity: >90%)
Related Product Information for METTL1/WDR4 recombinant protein
WDR4 (WD Repeat-Containing Protein 4), also known as TRM82, is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. METTL1 (MethyltransferaseLike Protein 1), also known as TRM8, contains a conserved S-adenosylmethionine-binding motif and is inactivated by the protein kinase B (PKB) and ribosomal S6 kinase (RSK) under growth factor stimulation. WRD4 and METTL1 that form a complex are required for tRNA (guanine(46)-N(7))-methyltransferase activity. More than 90 different modified nucleosides have been identified in tRNA. Among the tRNA modifications, the 7-methylguanosine (m7G) modification is found widely in eubacteria, eukaryotes, and a few archaea. In most cases, the m7G modification occurs at position 46 in the variable region and is a product of tRNA (m7G46) methyltransferase. The m7G46 modification forms a tertiary base pair with C13-G22 and stabilizes the tRNA structure.
Product Categories/Family for METTL1/WDR4 recombinant protein
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Product Notes
The METTL1/WDR4 (Catalog #AAA60403) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "METTL1/WDR4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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