IP (Immunoprecipitation)
(METTL1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-METTL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-METTL1 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
Mouse anti-Human METTL1 Monoclonal Antibody | anti-METTL1 antibody
Anti-METTL1 Antibody, Mouse Monoclonal
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human
Applications
Immunoprecipitation, ELISA, ELISA, Western Blot
Purity
Protein A
Synonyms
METTL1, Antibody; Anti-METTL1 Antibody, Mouse Monoclonal; METTL1 Antibody, Mouse MAb; methyltransferase like 1; Anti-C12orf1 Antibody; Anti-TRM8 Antibody; Anti-TRMT8 Antibody; Anti-YDL201w Antibody; anti-METTL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
9C2F2
Specificity
Human METTL1.
No cross-reactivity in ELISA with E Coli cell lysate
No cross-reactivity in ELISA with E Coli cell lysate
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-METTL1 antibody
IP (Immunoprecipitation), ELISA, ELISA, WB (Western Blot)
Immunogen
Recombinant Human METTL1 protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (rh METTL1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(METTL1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-METTL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-METTL1 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
IP (Immunoprecipitation)
(METTL1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate2 uL anti-METTL1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-METTL1 mouse monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 34 kDaObserved band size: 34 kDa)
WB (Western Blot)
(Anti-METTL1 mouse monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa)
WB (Western Blot)
(Anti-METTL1 mouse monoclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:31 kDaObserved band size:31 kDa)
Related Product Information for anti-METTL1 antibody
tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
Product Categories/Family for anti-METTL1 antibody
References
Bahr, A.et al., 1999, Genomics. 57 (3):424-8. Wikman, H. et al., 2005, Genes Chromosomes Cancer. 42 (2):193-9. Cartlidge, RA. et al., 2005, EMBO J. 24 (9):1696-705.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,423 Da
NCBI Official Full Name
tRNA (guanine-N(7)-)-methyltransferase isoform a
NCBI Official Synonym Full Names
methyltransferase like 1
NCBI Official Symbol
METTL1
NCBI Official Synonym Symbols
TRM8; TRMT8; C12orf1; YDL201w
NCBI Protein Information
tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
UniProt Protein Name
tRNA (guanine-N(7)-)-methyltransferase
UniProt Gene Name
METTL1
UniProt Entry Name
TRMB_HUMAN
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Product Notes
The METTL1 mettl1 (Catalog #AAA255421) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-METTL1 Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the METTL1 mettl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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