Mouse MMP-9 ELISA Kit | MMP-9 elisa kit

MMP-9 Mouse ELISA Kit

Cat. Num. AAA58931

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: MMP-9; N/A; MMP-9 Mouse ELISA Kit; Mouse MMP-9; MMP-9 elisa kit

• Reactivity: Mouse
• Assay Type: Sandwich
• Samples: Mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 20 pg/ml

• Applicable Applications for MMP-9 elisa kit: ELISA
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Standard Curve (Sample)

Related Product Information for MMP-9 elisa kit

Background: Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is a member of the matrix metalloproteinase (MMP) family. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, intracerebral hemorrhage, and metastasis. MMP-9 is produced by normal alveolar macrophages and granulocytes. Sundstrom et al. (2004) suggested that plasma MMP9 level may be a marker for cardiac extracellular matrix degradation, a process involved in left ventricular remodeling.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-9 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-9 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985.2
• NCBI GenBank Nucleotide #: NM_004994.2
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• Molecular Weight: 78,458 Da
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN