Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Published customer image:RPE conjugated Mouse anti Canine CD4 antibody, clone YKIC302.9used for the assessment of CD4 levels on canine cells by flow cytometry.Image caption:Immunophenotypic profile of tumor infiltrating lymphocyte in canine mammary carcinomas. Analysis of tumor infiltrating T-cells, B-lymphocytes and T-cell subsets from MC-BMT or MC (A), further subcategorized according to the absence (-) or presence (+) of lymph node metastasis (-) (B). Lymphocyte populations and subsets were identified by flow cytometric immunostaining as described in Material and Methods. Data were expressed as percentage of positive cells within gated lymphocytes and CD4+/CD8+ T-cell ratio. Significant differences at p < 0.05 are highlighted by asterisk.)

Application Data (Published customer image:Pacific Blue conjugated Mouse anti Canine CD4 antibody, clone YKIX302.9 used for evaluation of CD4 expression on canine cells by flow cytometry.Image caption:CD11b+CD14-MHCII- cells suppress T cell proliferation.Facs sorted CD11b+CD14-MHCII- cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4+ and CD8+ T cells isolated from a healthy dog for 72 hrs. No stimulated cells were used as negative control. Proliferative responses were measured by 3H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean +/- SEM are shown.From: Goulart MR, Pluhar GE, Ohlfest JR (2012)Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer.)

Application Data (Figure A. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b FITC isotype control . Figure B. Purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 PE and Rat anti Canine CD4 FITC . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. A488 conjugated Rat anti Canine CD4 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649 . Figure B. A488 conjugated Rat anti Canine CD4 and purified Mouse anti Canine CD8b detected with Goat anti Mouse IgG1 DyLight 649 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated Mouse anti Canine CD5 and A647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Canine CD5 and A647 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat IgG2a isotype control . Figure B. A647 conjugated Rat anti Canine CD4 and RPE conjugated Rat anti Canine CD5 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse IgG1 isotype control . Figure B. RPE conjugated Rat anti Canine CD4 and FITC conjugated Mouse anti Canine CD3 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Mouse anti Canine CD3 and RPE conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on mononuclear cells.)

Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Canine CD5 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Canine CD5 and Alexa Fluor 488 conjugated Rat anti Canine CD4 . All experiments performed on red cell lysed canine blood gated on lymphocytes in the presence of 10% canine serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Dog CD8 and RPE conjugated Rat anti Dog CD4 . All experiments performed on red cell lysed canine blood gated on lymphoid cells in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.)

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CD4, Monoclonal Antibody (Cat# AAA12278)

Full Name

RAT ANTI DOG CD4: APC

Reactivity

Dog

Applications

Flow Cytometry (FC/FACS)

Pricing

$750/100 Tests $1,450/2x100 Tests $2,110/3x100 Tests $2,735/4x100 Tests $3,110/5x100 Tests

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Niemann Pick C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Niemann Pick C1 on SiHa cell lysate using anti-Niemann Pick C1 antibody at 1/500 dilution.)

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Niemann Pick C1, Monoclonal Antibody (Cat# AAA30458)

Full Name

Niemann Pick C1 Antibody

Gene Names

NPC1; NPC

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

WB (Western Blot) (TIMM9 monoclonal antibody Western Blot analysis of TIMM9 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged TIMM9 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody.Lane 1: TIMM9 transfected lysate (10.4kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)

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TIMM9, Monoclonal Antibody (Cat# AAA24059)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A)

Reactivity

Human, Mouse, Rat

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

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WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged HOXC12 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in Jurkat.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in Raw 264.7.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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HOXC12, Monoclonal Antibody (Cat# AAA24831)

Full Name

HOXC12 (Homeobox Protein Hox-C12, Homeobox Protein Hox-3F, HOC3F, HOX3F) (Biotin)

Gene Names

HOXC12; HOX3; HOC3F; HOX3F

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in HeLa.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged EIF2S2 is 0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2S2 on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF2S2 expression in transfected 293T cell line by EIF2S2 monoclonal antibody. Lane 1: EIF2S2 transfected lysate (38.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in NIH/3T3.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in Raw 264.7.)

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EIF2S2, Monoclonal Antibody (Cat# AAA25087)

Full Name

EIF2S2 (EIF2B, Eukaryotic Translation Initiation Factor 2 Subunit 2, Eukaryotic Translation Initiation Factor 2 Subunit beta, DKFZp686L18198, EIF2Beta, MGC8508) (FITC)

Gene Names

EIF2S2; EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody. Lane 1: ACY1 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml])

IP (Immunoprecipitation) (Immunoprecipitation of ACY1 transfected lysate using ACY1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ACY1 rabbit polyclonal antibody.)

WB (Western Blot) (ACY1 monoclonal antibody Western Blot analysis of ACY1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (70.62kD).)

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ACY1, Monoclonal Antibody (Cat# AAA25599)

Full Name

ACY1 (Aminoacylase 1, Aminoacylase-1, ACY-1, N-acyl-L-amino-acid Amidohydrolase) (PE)

Gene Names

ACY1; ACY-1; ACY1D; HEL-S-5

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

Application Data (Detection limit for recombinant GST tagged STIP1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of STIP1 transfected lysate using STIP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with STIP1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3ug/ml].)

WB (Western Blot) (Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody Lane 1: STIP1 transfected lysate (62.6kD). Lane 2: Non-transfected lysate.)

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STIP1, Monoclonal Antibody (Cat# AAA25855)

Full Name

STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (PE)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)

WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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RUNX2, Monoclonal Antibody (Cat# AAA26111)

Full Name

RUNX2 (Runt-Related Transcription Factor 2, AML3, CBFA1, CCD, CCD1, MGC120022, MGC120023, OSF2, PEA2aA, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1) (APC)

Gene Names

RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3. Western Blot analysis of AKT2 expression in PC-12.)

WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3. Western Blot analysis of AKT2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3 Western Blot analysis of AKT2 expression in Jurkat (Cat # L017V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT2, Monoclonal Antibody (Cat# AAA26367)

Full Name

AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (FITC)

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Desmoglein 2 on A431 cell using anti-Desmoglein 2 antibody at 1/500 dilution.)

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Desmoglein 2, Monoclonal Antibody (Cat# AAA30463)

Full Name

Desmoglein 2 Antibody

Gene Names

DSG2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12123)

Full Name

RAT ANTI MOUSE CD206

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP)

Pricing

$450/0.1 mg $865/2x0.1 mg $1,255/3x0.1 mg $1,625/4x0.1 mg $1,775/5x0.1 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Azide Free)

Application Data (Staining of human peripheral blood monocytes using Mouse anti Human CD38:FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:RPE-Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD38:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD38:Alexa Fluor 647)

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CD38, Monoclonal Antibody (Cat# AAA12062)

Full Name

MOUSE ANTI HUMAN CD38

Gene Names

CD38; T10; ADPRC 1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$250/0.02 mg $475/2x0.02 mg $685/3x0.02 mg $870/5x0.02 mg $885/4x0.02 mg

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

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CD4, Monoclonal Antibody (Cat# AAA11900)

Full Name

RAT ANTI MOUSE CD4:Low Endotoxin

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Functional Assays (FN)

Pricing

$865/0.5 mg $1,675/2x0.5 mg $2,440/3x0.5 mg $3,160/4x0.5 mg $3,630/5x0.5 mg

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Application Data (Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)

Application Data (Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.)

Application Data (Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm)

Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm)

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CD68, Monoclonal Antibody (Cat# AAA12104)

Full Name

RAT ANTI MOUSE CD68:Biotin

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

Flow cytometry (FC/FACS)*

Pricing

$260/0.025 mg $495/2x0.025 mg $715/3x0.025 mg $920/5x0.025 mg $925/4x0.025 mg

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12120)

Full Name

RAT ANTI MOUSE CD206:FITC

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow cytometry (FC/FACS)*

Pricing

$345/0.05 mg $660/2x0.05 mg $960/3x0.05 mg $1,240/4x0.05 mg $1,295/5x0.05 mg

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12117)

Full Name

RAT ANTI MOUSE CD206:Biotin

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow cytometry (FC/FACS)*

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,980/4x0.1 mg $2,195/5x0.1 mg

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Application Data (Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)

Application Data (Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.)

Application Data (Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm)

Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm)

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CD68, Monoclonal Antibody (Cat# AAA12108)

Full Name

RAT ANTI MOUSE CD68:RPE

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

Flow cytometry (FC/FACS)*

Pricing

$645/100 Tests $1,245/2x100 Tests $1,815/3x100 Tests $2,350/4x100 Tests $2,650/5x100 Tests

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Medium power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 750)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647)

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CD3, Monoclonal Antibody (Cat# AAA12175)

Full Name

RAT ANTI MOUSE CD3

Gene Names

Cd3e; CD3; T3e; AI504783; CD3epsilon

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa FluorR 750)

Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa FluorR 647)

Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3):)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): Biotin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3)::Alexa FluorR 488)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3):FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low (AAA12243))

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CD3, Monoclonal Antibody (Cat# AAA12243)

Full Name

RAT ANTI MOUSE CD3:Low Endotoxin

Gene Names

Cd3e; CD3; T3e; AI504783; CD3epsilon

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$865/0.5 mg $1,675/2x0.5 mg $2,440/3x0.5 mg $3,160/4x0.5 mg $3,630/5x0.5 mg

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405 (AAA11938A405))

Application Data (Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

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CD4, Monoclonal Antibody (Cat# AAA11938)

Full Name

RAT ANTI MOUSE CD4

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$560/0.25 mg $1,080/2x0.25 mg $1,570/3x0.25 mg $2,035/4x0.25 mg $2,275/5x0.25 mg

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

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CD4, Monoclonal Antibody (Cat# AAA12091)

Full Name

RAT ANTI MOUSE CD4

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:RPE)

Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody , red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)

Application Data (Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody in red and with Mouse anti Human CD21 antibody in red. Nuclei are stained blue using DAPI. Merged image on right)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)

Application Data (Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)

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CD21, Monoclonal Antibody (Cat# AAA11922)

Full Name

MOUSE ANTI HUMAN CD21

Gene Names

CR2; CR; C3DR; CD21; CVID7; SLEB9

Reactivity

Bovine, Cat, Goat, Mink, Sheep

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Pricing

$655/2 mL $1,265/2x2 mL $1,840/3x2 mL $2,385/4x2 mL $2,690/5x2 mL

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)

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CD4, Monoclonal Antibody (Cat# AAA11926)

Full Name

MOUSE ANTI HUMAN CD4

Gene Names

CD4; CD4mut

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$410/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,480/4x0.1 mg $1,600/5x0.1 mg

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)

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CD4, Monoclonal Antibody (Cat# AAA12209)

Full Name

MOUSE ANTI HUMAN CD4

Gene Names

CD4; CD4mut

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : Biotin . Permeabilised with Leucoperm)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD107b: FITC)

Application Data (Staining of mouse peritoneal macrophages with Rat anti MouseCD107b : Alexa Fluor 647 . Permeabilised with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD1076 following permeabilsation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : FITC following permeabilisation with Leucoperm)

Application Data (Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Goat anti Rat IgG:Dylight®)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : Alexa Fluor 488 . Permeabilised with Leucoperm)

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CD107b, Monoclonal Antibody (Cat# AAA11959)

Full Name

RAT ANTI MOUSE CD107b

Gene Names

Lamp2; Mac3; LGP-B; CD107b; Lamp-2; Lamp II; Lamp-2a; Lamp-2b; Lamp-2c

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)

Pricing

$410/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,480/4x0.1 mg $1,600/5x0.1 mg

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Application Data (Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE. Permeabilised with Leucoperm (Fix & Perm))

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power)

Application Data (Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm)

Application Data (Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)

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CD68, Monoclonal Antibody (Cat# AAA12150)

Full Name

MOUSE ANTI RAT CD68:RPE

Applications

Flow cytometry (FC/FACS)*

Pricing

$740/100 Tests $1,430/2x100 Tests $2,085/3x100 Tests $2,700/4x100 Tests $3,065/5x100 Tests

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Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa FluorR 647 . following permeablisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: RPE . following permeablisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa FluorR 488 . following permeablisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68 CD68 , following permeablisation with Leucoperm)

Application Data (Staining of permeabilised mouse peritoneal macrophages cells with Rat anti Mouse CD68 visualised with F(ab')2 Goat anti Rat IgG:FITC (Mouse adsorbed))

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:FITC . following permeablisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD68:Biotin .following permeablisation with Leucoperm)

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CD68, Monoclonal Antibody (Cat# AAA12245)

Full Name

RAT ANTI MOUSE CD68:Endotoxin Low

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*

Pricing

$865/0.5 mg $1,675/2x0.5 mg $2,440/3x0.5 mg $3,160/4x0.5 mg $3,630/5x0.5 mg

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IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Skeletal Muscle stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

WB (Western Blot) (Western Blot of hSKM Cell Lysate using Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (MSA/953))

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Actin, Monoclonal Antibody (Cat# AAA13822)

Full Name

Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human, Rabbit, Rat

Applications

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 40ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody. Lane 1: MPG transfected lysate (32.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MPG monoclonal antibody Western Blot analysis of MPG expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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MPG, Monoclonal Antibody (Cat# AAA24574)

Full Name

MPG (DNA-3-methyladenine Glycosylase, 3-alkyladenine DNA Glycosylase, 3-methyladenine DNA Glycosidase, ADPG, N-methylpurine-DNA Glycosylase, AAG, ANPG, MID1) APC

Gene Names

MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HNRPM is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNRPM on HepG2 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HNRPM on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3ug/ml.)

WB (Western Blot) (HNRPM monoclonal antibody. Western Blot analysis of HNRPM expression in NIH/3T3.)

WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in HepG2.)

WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.3kD).)

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HNRNPM, Monoclonal Antibody (Cat# AAA24830)

Full Name

HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M, hnRNP M, HNRPM, NAGR1, DKFZp547H118) (Biotin)

Gene Names

HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.)

IHC (Immunohistchemistry) (Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

WB (Western Blot) (Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml)

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CD80, Monoclonal Antibody (Cat# AAA11001)

Full Name

CD80 Antibody [12D9]

Gene Names

CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Protein A purified

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])

WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody.Lane 1: ASNA1 transfected lysate (38.8kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

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ASNA1, Monoclonal Antibody (Cat# AAA24057)

Full Name

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40)

Gene Names

ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I; hASNA-I

Reactivity

Human, Rat

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in different cell lines.)

WB (Western Blot) (USF2 monoclonal antibody (M02), clone 5F2. Western Blot analysis of USF2 expression in Hela S3 NE (Cat # L013V3).)

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USF2, Monoclonal Antibody (Cat# AAA26361)

Full Name

USF2 (Upstream Transcription Factor 2, c-fos Interacting, FIP, bHLHb12) (FITC)

Gene Names

USF2; FIP; bHLHb12

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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PGRMC2, Monoclonal Antibody (Cat# AAA24312)

Full Name

PGRMC2 (Membrane-associated Progesterone Receptor Component 2, Progesterone Membrane-binding Protein, Steroid Receptor Protein DG6, DG6, PMBP) (AP)

Gene Names

PGRMC2; DG6; PMBP

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJB6 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody. Lane 1: DNAJB6 transfected lysate (27KD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DNAJB6 monoclonal antibody Western Blot analysis of DNAJB6 expression in Jurkat.)

WB (Western Blot) (DNAJB6 monoclonal antibody Western Blot analysis of DNAJB6 expression in C32.)

WB (Western Blot) (Western Blot detection against Immunogen (52.25kD).)

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DNAJB6, Monoclonal Antibody (Cat# AAA25080)

Full Name

DNAJB6 (HSJ2, MRJ, MSJ1, DnaJ Homolog Subfamily B Member 6, HHDJ1, Heat Shock Protein J2, HSJ-2, MSJ-1, MGC1152, MGC117297, DKFZp566D0824, FLJ42837) (FITC)

Gene Names

DNAJB6; DJ4; MRJ; DnaJ; HSJ2; HHDJ1; HSJ-2; MSJ-1; LGMD1D; LGMD1E; LGMDD1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ABL2, Monoclonal Antibody (Cat# AAA25592)

Full Name

ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (PE)

Gene Names

ABL2; ARG; ABLL

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)

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DLX5, Monoclonal Antibody (Cat# AAA25079)

Full Name

DLX5 (Homeobox Protein DLX-5) (FITC)

Gene Names

DLX5; SHFM1D

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)

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SNRPA, Monoclonal Antibody (Cat# AAA25847)

Full Name

SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A) (PE)

Gene Names

SNRPA; U1A; Mud1; U1-A

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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HNF4A, Monoclonal Antibody (Cat# AAA24829)

Full Name

HNF4A (Hepatocyte Nuclear Factor 4-alpha, HNF-4-alpha, Transcription Factor HNF-4, Nuclear Receptor Subfamily 2 Group A Member 1, Transcription Factor 14, HNF4, NR2A1, TCF14) (Biotin)

Gene Names

HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)

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ACTB, Monoclonal Antibody (Cat# AAA25597)

Full Name

ACTB (Actin, Cytoplasmic 1, Beta-actin) (PE)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431 (Cat # L015V1).)

WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26621)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (PE)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution. Positive control: Lane 1: SiHa Lane 2: Mouse colon Lane 3: Rat liver)

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Glutathione Synthetase, Monoclonal Antibody (Cat# AAA30462)

Full Name

Glutathione Synthetase Antibody

Gene Names

GSS; GSHS; HEL-S-64p; HEL-S-88n

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (CBS monoclonal antibody. Western Blot analysis of CBS expression in MCF-7.)

WB (Western Blot) (CBS monoclonal antibody, Western Blot analysis of CBS expression in HeLa.)

WB (Western Blot) (Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 124401. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CBS is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CBS transfected lysate using 124401and Protein A Magnetic Bead and immunoblotted with CBS rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of formalin-fixed paraffin-embedded human hepatocellular carcinoma using 124401 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of CBS expression in transfected 293T cell line by 124401. Lane 1: CBS transfected lysate (61kD). Lane 2: Non-transfected lysate.)

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CBS, Monoclonal Antibody (Cat# AAA25340)

Full Name

CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) (HRP)

Gene Names

CBS; HIP4

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (SPRY2 monoclonal antibody Western Blot analysis of SPRY2 expression in C32)

Application Data (Detection limit for recombinant GST tagged SPRY2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (60.76kD).)

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SPRY2, Monoclonal Antibody (Cat# AAA25852)

Full Name

SPRY2 (Protein Sprouty Homolog 2, Spry-2, hSPRY2) (PE)

Gene Names

SPRY2; IGAN3; hSPRY2

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in PC-12.)

WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9 Western Blot analysis of AKT2 expression in Jurkat (Cat # L017V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT2, Monoclonal Antibody (Cat# AAA26364)

Full Name

AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (FITC)

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining TRK fused gene in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRK fused gene in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRK fused gene in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5, 000 dilution. Positive control: Lane 1: 293T Lane 2: Hela)

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TRK Fused Gene, Monoclonal Antibody (Cat# AAA30204)

Full Name

TRK Fused Gene Antibody

Gene Names

TFG; TF6; HMSNP; SPG57; TRKT3

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Application Data (Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.Image caption:ERp57 overexpression reduces axonal)

Application Data (Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for identification of CD11b expressing cells in murine sciatic nerves by immunofluorescence on cryosections.Image caption:Overexpression of ERp57 in transgeni)

Application Data (Published customer image:AlexaFluor647 conjugated Rat ati mouse CD11b antibody, clone M1/70.15 used for the evaluation of CD11b expression on immortalized murine microglia by flow cytometry.expressionImage caption:IMG cells display)

Application Data (Published customer image:Rat anti mouse CD11b antibody, clone M1/70.15 used for the assessment of CD11b expression by activated N9 microglial cells using immunofluorescence.Image caption:Localization of CD11b and p-STAT3 immunoreactiv)

Application Data (Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for the localization of microglia in murine brain tissue sections by immunofluorescenceImage caption:HCT1026 inhibits MPTP-induced increased Mac-1, PHOX, iNOX)

Application Data (Published customer image:Rat anti Mouse CD11b antibody, clone M1/70.15 used for the localization of microglia in murine brain tissue sections by immunofluorescenceImage caption:HCT1026 inhibits MPTP-induced increased reactive Mac-1+ m)

Application Data (Published customer image:Rat anti MouseCD11b antibody, clone M1/70.15 used to identify murine microglia in vitro by immunofluorescence.Image caption:Immunocytochemical characterisation of cultures of mouse microglia. Cultures of micro)

Application Data (Figure A. FITC conjugated hamster anti mouse CD11c and Alexa647 conjugated rat IgG2b isotype control . Figure B. FITC conjugated hamster anti mouse CD11c and AlexaFluor647 conjugated rat anti mouse CD11b . All)

IF (Immunofluorescence) (Immunofluorescence staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody , green in A and Rat anti Mouse CD8 antibody , red in B, Merged image in C)

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CD11b, Monoclonal Antibody (Cat# AAA12285)

Full Name

Rat anti Mouse CD11b:Amethyst Orange

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Reactivity

Mouse

Applications

Flow Cytometry (FC/FACS)

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$585/0.1 mg $1,130/2x0.1 mg $1,640/3x0.1 mg $2,125/4x0.1 mg $2,380/5x0.1 mg

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WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in NIH/3T3.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged ACP1 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody. Lane 1: ACP1 transfected lysate (18kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in Raw 264.7.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (43.12kD).)

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ACP1, Monoclonal Antibody (Cat# AAA25595)

Full Name

ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1, PTPase, Adipocyte Acid Phosphatase) (PE)

Gene Names

ACP1; HAAP; LMW-PTP

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562 (Cat # L009V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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TCF7L2, Monoclonal Antibody (Cat# AAA26619)

Full Name

TCF7L2 (Transcription Factor 7-like 2 (T-Cell Specific, HMG-Box), TCF-4, TCF4) (PE)

Gene Names

TCF7L2; TCF4; TCF-4

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 750)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19)

Application Data (Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:APC)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:FITC)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE)

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CD19, Monoclonal Antibody (Cat# AAA12028)

Full Name

RAT ANTI MOUSE CD19:RPE

Gene Names

Cd19; AW495831

Applications

Flow cytometry (FC/FACS)

Pricing

$585/100 Tests $1,130/2x100 Tests $1,640/3x100 Tests $2,125/4x100 Tests $2,380/5x100 Tests

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.