Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution.)
ELISA (Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [9E4]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [9E4]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.Green: VISTA Antibody [9E4]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody. Lane 1: NEK10 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)
WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5, 000 dilution.)
ELISA (Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [8E11]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [8E11]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.Green: VISTA Antibody [8E11]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)
Application Data (Published customer image:Insert meta-description and applicationsImage caption:Insert image caption)
Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power.)
Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power.)
Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power.)
Application Data (Figure A. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat anti Mouse CD301 . All experiments performed on mouse peritoneal cells gated on live single monocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)
WB (Western Blot) (Detection of human CD40 by western blot. Samples: Whole cell lysate (50 ug) from Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3 .)
IP (Immunoprecipitation) (Detection of human CD40 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used for IP at 5 ul per reaction. CD40 was also immunoprecipitated by rabbit anti-CD40 recombinant monoclonal antibody (BL-790-1G12). For blotting immunoprecipitated CD40, AAA23814 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human CD40 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD40 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD40 by immunocytochemistry. Sample: FFPE section of Raji cells. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CD40 (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (AAA23814) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with Syntrophin alpha 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Syntrophin alpha 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syntrophin alpha 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syntrophin alpha 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Syntrophin alpha 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Syntrophin alpha 1 on SiHa cells lysates using anti-Syntrophin alpha 1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Detection of human and mouse beta-Catenin by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, LNCaP, K-562, GaMG, Jurkat, A-549, MCF-7, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Catenin recombinant monoclonal antibody (AAA23827 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistchemistry) (Detection of human beta Catenin in FFPE prostate carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of mouse beta Catenin in FFPE plasmacytoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human beta Catenin in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human beta Catenin in FFPE OVCAR-3 cells by ICC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GABBR1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody. Lane 1: GABBR1 transfected lysate (95kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GABBR1 monoclonal antibody, Western Blot analysis of GABBR1 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])
WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with GLUT1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining GLUT1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GLUT1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GLUT1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-GLUT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GLUT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GLUT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GLUT1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GLUT1 on different cell lysates using anti-GLUT1 antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Jurkat Lane 4: NIH/3T3)
WB (Western Blot) (Detection of human PCNA by western blot. Samples: Whole cell lysate (50 ug) from A-549, Jurkat, HEK293T, RKO, K-562, MCF-7, HeLa, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH .)
IHC (Immunohistochemistry) (Detection of human PCNA in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of human PCNA by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human PCNA by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human PCNA by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human PCNA (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-PCNA recombinant monoclonal antibody (AAA23821) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western Blot detection against Immunogen (84.7kD).)
WB (Western Blot) (Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LCK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LCK transfected lysate using LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody. Lane 1: LCK transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LCK monoclonal antibody Western Blot analysis of LCK expression in Jurkat.)
WB (Western Blot) (FLJ20097 monoclonal antibody, Western Blot analysis of FLJ20097 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ20097 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in NIH/3T3.)
WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in human pancreas.)
WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody.Lane 1: HADH transfected lysate (34.3kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)
WB (Western Blot) (Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LDB3 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody. Lane 1: LDB3 transfected lysate (31kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (57.24kD).)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HeLa.)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged FLJ23356 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ23356 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody. Lane 1: FLJ23356 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FLJ23356 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody. Lane 1: CRKL transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with PTK2 rabbit purified polyclonal 1:600 and CRKL mouse monoclonal antibody 1:100. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with GAB1 rabbit purified polyclonal 1:1200 and CRKL mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged CRKL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CRKL on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
ICC (Immunocytochemistry) (ICC staining HSPA14 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HSPA14 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HSPA14 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSPA14 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HSPA14 on different lysates using anti-HSPA14 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: COS-1)
PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (AP)
WB (Western Blot) (KHDRBS1 monoclonal antibody Western Blot analysis of KHDRBS1 expression in A-431)
Application Data (Detection limit for recombinant GST tagged KHDRBS1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KHDRBS1 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody. Lane 1: KHDRBS1 transfected lysate (41kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)
WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ALB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ALB transfected lysate using ALB monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALB rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (ALB monoclonal antibody Western Blot analysis of ALB expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (90.75kD).)
WB (Western Blot) (Detection of mouse CDK1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 and F9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin .)
WB (Western Blot) (Detection of human CDK1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, U2OS, K-562, HEK293T, A-549, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin .)
IP (Immunoprecipitation) (Detection of human CDK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1) used for IP at 20 ul/mg lysate. CDK1 was also immunoprecipitated by rabbit anti-CDK1 antibodies . For blotting immunoprecipitated CDK1, AAA23826 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human CDK1 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CDK1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CDK1 in FFPE MOLT4 cells by ICC. Antibody: Rabbit anti-CDK1 recombinant monoclonal antibody (AAA23826 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CDK1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CDK1 recombinant monoclonal (AAA23826 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (SF3B2 monoclonal antibody . Western Blot analysis of SF3B2 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SF3B2 on HeLa cell. [antibody concentration 60ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SF3B2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml].)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in NIH/3T3.)
WB (Western Blot) (SF3B2 monoclonal antibody Western Blot analysis of SF3B2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (32.05kD).)
WB (Western Blot) (Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA2 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FOXA2 transfected lysate using FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody Lane 1: FOXA2 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA2 monoclonal antibody Western Blot analysis of FOXA2 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (36.19kD).)
WB (Western Blot) (CSK monoclonal antibody. Western Blot analysis of CSK expression in Hela NE.)
WB (Western Blot) (Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody. Lane 1: CSK transfected lysate (50.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CSK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CSK transfected lysate using CSK monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CSK rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody. Lane 1: ARNT transfected lysate (86.6kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ARNT is ~0.03ng/ml as a capture antibod)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARNT monoclonal antibody Western Blot analysis of ARNT expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
WB (Western Blot) (Detection of human and mouse USP7/HAUSP by western blot. Samples: Whole cell lysate (50 ug) from RKO, HEK293T, K-562, Hep-G2, MCF-7, Jurkat, HeLa, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-eEF2 .)
IP (Immunoprecipitation) (Detection of human USP7/HAUSP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used for IP at 20 ul/mg lysate. USP7/HAUSP was also immunoprecipitated by rabbit anti-USP7 antibody For blotting immunoprecipitated USP7/HAUSP, AAA23820 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry) (Detection of mouse USP7/HAUSP by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human USP7/HAUSP by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human USP7/HAUSP by immunocytochemistry. Sample: FFPE section of OVCAR-4 cells. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human USP7/HAUSP (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-USP7/HAUSP recombinant monoclonal antibody (AAA23820) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.