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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)
WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)
IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)
WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2.)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1, 000 dilution. Positive control�� Line1: MOLT-4 Line2: LNCaP Line3: Hela Line4: HEK-293 Line5: Jurkat)
WB (Western Blot) (Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 1: NIH-3T3 Lane 2: Rat kidney tissue Lane 3: Mouse testis tissue)
WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)
IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with SOX9 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SOX9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SOX9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SOX9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SOX9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SOX9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SOX9 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)
WB (Western Blot) (EIF4G1 monoclonal antibody, Western Blot analysis of EIF4G1 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged EIF4G1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF4G1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody. Lane 1: EIF4G1 transfected lysate (70.95kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (ACSL5 monoclonal antibody, Western Blot analysis of ACSL5 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged ACSL5 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ACSL5 transfected lysate using ACSL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ACSL5 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACSL5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACSL5 expression in transfected 293T cell line by ACSL5 monoclonal antibody. Lane 1: ACSL5 transfected lysate (82.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.67kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MAPK14 and MKNK2 HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and anti-MKNK2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
WB (Western Blot) (Western Blot analysis of MKNK2 expression in transfected 293T cell line by MKNK2 monoclonal antibody (M07), clone 2A10.Lane 1: MKNK2 transfected lysate (46.7 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in PC-12 (Cat # L012V1).)
WB (Western Blot) (MKNK2 monoclonal antibody (M07), clone 2A10. Western Blot analysis of MKNK2 expression in NIH/3T3 (Cat # L018V1).)
Application Data (Detection limit for recombinant GST tagged MKNK2 is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (MKNK2 monoclonal antibody (M07), clone 2A10 Western Blot analysis of MKNK2 expression in K-562 (Cat # L009V1).)
Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)
WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RAD51C is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody. Lane 1: RAD51C transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RAD51C monoclonal antibody. Western Blot analysis of RAD51C expression in 293.)
WB (Western Blot) (RAD51C monoclonal antibody, Western Blot analysis of RAD51C expression in HeLa.)
Application Data (IMAB027 inhibited the tumor growth of OV90 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 50.7% at 5 mpk at D34.)
Application Data (Anti-CLDN6 Reference Antibody (IMAB027)by huCLDN6-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 1 nM.)
Application Data (Anti-CLDN6 Reference Antibody (IMAB027)-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell. The max induction fold was approximately 14.16)
FCM (Flow Cytometry) (Human CLDN6 CHO cells were stained with Anti-CLDN6 Reference Antibody (IMAB027) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC199=4.342 nM)
Application Data (Immobilized human CLDN6 VLP at 8 ug/mL can bind Anti-CLDN6 Reference Antibody (IMAB027), EC50=0.001599ug/mL)
Application Data (The purity of Anti-CLDN6 Reference Antibody (IMAB027)is more than 98.41% ,determined by SEC-HPLC.)
SDS-PAGE (Anti-CLDN6 Reference Antibody (IMAB027) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in HeLa.)
WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged EIF2S2 is 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2S2 on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF2S2 expression in transfected 293T cell line by EIF2S2 monoclonal antibody. Lane 1: EIF2S2 transfected lysate (38.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in NIH/3T3.)
WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in Raw 264.7.)
WB (Western Blot) (SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)
Application Data (Detection limit for recombinant GST tagged SSH3 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1-10ug/ml])
WB (Western Blot) (Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody. Lane 1: SSH3 transfected lysate (73kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate (60.3 KDa).Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628).U-2 OS cells were treated by TSA (1 uM) at 37? for 18 hours(left). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628).NIH-3T3 cells were treated by TSA (1 uM) at 37? for 18 hours(left). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628).C6 cells were treated by TSA (1 uM) at 37? for 18 hours(left). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human appendix using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)
DB (Dot Blot) (Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K5 antibody (AAA28628) at 1:1000 dilution.)
WB (Western Blot) (Western blot analysis of various lysates using Acetyl-Histone H4-K5 Rabbit mAb (AAA28628) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Villin1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Villin1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Villin1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Villin1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Villin1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Villin1 on different tissue lysates using anti-Villin1 antibody at 1/500 dilution. Positive control: Lane 1: Mouse colon Lane 2: Human small intestine Lane 3: Human colon Lane 4: Rat kidney)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with AGR2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining AGR2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AGR2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AGR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-AGR2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of AGR2 on MCF-7 cells lysates using anti-AGR2 antibody at 1/1, 000 dilution.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SOX9 antibody . Western blot was performed from the immunoprecipitate using SOX9 antibody at a dilition of 1:1000.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SOX9 antibody . Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using SOX9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using SOX9 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using SOX9 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SOX9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)
IB (Immunoblot) (Figure 8. Immunoblotting results (R4A) on uterine artery and uterine corpus.)
IF (Immunofluorescence) (Figure 7. Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by R4A) and actin (green fluorescence).)
IHC (Immunohistchemistry) (Figure 6. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
IHC (Immunohistochemistry) (Figure 5. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
IHC (Immunohistochemistry) (Figure 4. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
IHC (Immunohistochemistry) (Figure 3. Immunohistochemistry of R4A on paraffin section of human smooth muscle.)
IHC (Immunohistochemistry) (Figure 2. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
IHC (Immunohistochemistry) (Figure 1. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)
WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)
WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa NE.)
Application Data (Detection limit for recombinant GST tagged CHEK2 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
WB (Western Blot) (AKAP10 monoclonal antibody Western Blot analysis of AKAP10 expression in Raw 264.7.)
WB (Western Blot) (RNAi Knockdown: Western blot analysis of AKAP10-over-expressing 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with Cat. # 123131. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged AKAP10 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase staining of AKAP10 on formalin-fixed paraffin-embedded human testis using Cat. # 123131 at 0.5ug/ml.)
WB (Western Blot) (Western Blot analysis of AKAP10 expression in transfected 293T cell line using Cat. # 123131: Lane 1: AKAP10 transfected lysate (73.8kD); Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
AKAP10 (A-kinase Anchor Protein 10, Mitochondrial, AKAP-10, Dual Specificity A Kinase-anchoring Protein 2, D-AKAP-2, Protein Kinase A-anchoring Protein 10, PRKA10) (HRP)
Gene Names
AKAP10; PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
Reactivity
Human, Mouse
Applications
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged ABL2 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (ABL2 monoclonal antibody (M09), clone 5C6 Western Blot analysis of ABL2 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (STAT5B monoclonal antibody (M03), clone 2D1 Western Blot analysis of STAT5B expression in HeLa (Cat # L013V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (CDK2 monoclonal antibody. Western Blot analysis of CDK2 expression in Jurkat.)
WB (Western Blot) (Western blot analysis of CDK2 over-expressed 293 cell line, cotransfected with CDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CDK2 is ~0.03ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of CDK2 expression in transfected 293T cell line by CDK2 monoclonal antibody. Lane 1: CDK2 transfected lysate (33.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CDK2 monoclonal antibody, Western Blot analysis of CDK2 expression in HL-60.)
WB (Western Blot) (Western Blot detection against Immunogen (35.42kD).)
Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (69.01kD).)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7 (Cat # L046V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)
WB (Western Blot) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (FITC)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged ACTN4 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody. Lane 1: ACTN4 transfected lysate (104.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in PC-12.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug Cyclin D1 antibody . Western blot was performed from the immunoprecipitate using Cyclin D1 antibody at a dilition of 1:1000.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using [KO Validated] Cyclin D1 Rabbit mAb at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Cyclin D1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
WB (Western Blot) (Western blot analysis of extracts from normal (control) and Cyclin D1 knockout (KO) HeLa cells, using Cyclin D1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293 cells with Glycogen synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Glycogen synthase in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Glycogen synthase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Glycogen synthase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glycogen synthase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Glycogen synthase on Hela lysates using anti- Glycogen synthase antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with ITGA2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ITGA2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ITGA2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ITGA2 on A431 cells lysates using anti-ITGA2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (EIF5 monoclonal antibody, Western Blot analysis of EIF5 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged EIF5 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF5 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF5 expression in transfected 293T cell line by EIF5 monoclonal antibody. Lane 1: EIF5 transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (73.15kD).)
WB (Western Blot) (STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)
WB (Western Blot) (Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IP (Immunoprecipitation) (Immunoprecipitation of STK33 transfected lysate using STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25ug/ml].)
WB (Western Blot) (Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody. Lane 1: STK33 transfected lysate (57.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (82.65kD).)
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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