Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin)

Application Data (Staining of U937 cells with Mouse anti Human CD284:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 488)

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CD284, Monoclonal Antibody (Cat# AAA11902)

Full Name

MOUSE ANTI HUMAN CD284:Low Endotoxin

Gene Names

TLR4; TOLL; CD284; TLR-4; ARMD10

Applications

Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)

Pricing

$865/0.5 mg $1,675/2x0.5 mg $2,440/3x0.5 mg $3,160/4x0.5 mg $3,630/5x0.5 mg

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Application Data (Staining of rat thymus cells with Mouse anti Rat CD90 (THY1.1):Alexa Fluor 488)

Application Data (Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1):FITC)

Application Data (Staining of rat thymocytes with Mouse anti Rat CD90 (THY1.1): FITC)

Application Data (Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1): Low Endotoxin)

Application Data (Staining of rat thymocytes with Mouse anti Rat CD90)

Application Data (Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1))

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CD90, Monoclonal Antibody (Cat# AAA12158)

Full Name

MOUSE ANTI RAT CD90:FITC

Gene Names

Thy1; CD7

Applications

Flow cytometry (FC/FACS)

Pricing

$515/0.1 mg $990/2x0.1 mg $1,440/3x0.1 mg $1,865/4x0.1 mg $2,065/5x0.1 mg

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WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in PC-12.)

WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in NIH/3T3.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml])

Application Data (Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11 Western Blot analysis of HSPA1B expression in Hela S3 NE.)

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HSPA1B, Monoclonal Antibody (Cat# AAA25979)

Full Name

HSPA1B (Heat Shock 70kD Protein 1B, FLJ54328, HSP70-1B, HSP70-2, HSPA1A) (AP)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Applications

Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M04), clone 3D2 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA26491)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (HRP)

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:FITC (MCA88F))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:BIOTIN (MCA88B))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:AZIDE FREE (MCA88XZ))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:ALEXA 647 (MCA88A647))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA: RPE - Alexa Fluor® 647 (MCA88P647))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti human CD45RA (MCAP88))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA (MCAP88))

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CD45RA, Monoclonal Antibody (Cat# AAA26747)

Full Name

CD45RA (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC)

Reactivity

Human, Monkey

Applications

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$1,055/0.1 mL $2,045/2x0.1 mL $2,980/3x0.1 mL $3,865/4x0.1 mL $4,510/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with S100 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-S100 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- S100 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- S100 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- S100 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of S100 on mouse heart cells lysates using anti-S100 antibody at 1/500 dilution.)

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S100, Monoclonal Antibody (Cat# AAA30331)

Full Name

S100 Antibody

Gene Names

S100A1; S100; S100A; S100-alpha

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)

Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

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CD3, Monoclonal Antibody (Cat# AAA12156)

Full Name

MOUSE ANTI HUMAN CD3

Gene Names

CD3G; T3G; IMD17; CD3-GAMMA

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of VAX1 expression in transfected 293T cell line by VAX1 monoclonal antibody Lane 1: VAX1 transfected lysate (21kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in Raw 264.7.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in HepG2.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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VAX1, Monoclonal Antibody (Cat# AAA24700)

Full Name

VAX1 (Ventral Anterior Homeobox 1, MCOPS11) APC

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of NDUFV2 expression in transfected 293T cell line by NDUFV2 monoclonal antibody. Lane 1: NDUFV2 transfected lysate (Predicted MW: 27.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in K-562.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in NIH/3T3.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in Raw 264.7.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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NDUFV2, Monoclonal Antibody (Cat# AAA25468)

Full Name

NDUFV2 (NADH Dehydrogenase [Ubiquinone] Flavoprotein 2, Mitochondrial, NADH-ubiquinone Oxidoreductase 24kD Subunit) (HRP)

Gene Names

NDUFV2; CI-24k

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (IL20 monoclonal antibody, Western Blot analysis of IL20 expression in K-562.)

Application Data (Detection limit for recombinant GST tagged IL20 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of IL20 transfected lysate using IL20 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL20 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of IL20 expression in transfected 293T cell line by IL20 monoclonal antibody Lane 1: IL20 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (IL20 monoclonal antibody. Western Blot analysis of IL20 expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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Interleukin 20, Monoclonal Antibody (Cat# AAA25724)

Full Name

Interleukin 20 (Interleukin-20, IL-20, IL20, Cytokine Zcyto10, IL10D, MGC96907, UNQ852/PRO1801, ZCYTO10) (PE)

Gene Names

IL20; IL-20; IL10D; ZCYTO10

Reactivity

Human

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:RPE (MCA2872PE))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 488 (MCA2872A488))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 647 (MCA2872A647))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:FITC (MCA2872F)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872GA))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872T))

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CD49d, Monoclonal Antibody (Cat# AAA26748)

Full Name

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (FITC)

Reactivity

Rat

Applications

Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Purity

Purified by Protein G Affinity Chromatography

Pricing

$960/0.1 mL $1,860/2x0.1 mL $2,710/3x0.1 mL $3,515/4x0.1 mL $4,080/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of human liver using Ceruloplasmin Rabbit mAb (AAA28540,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse liver using Ceruloplasmin Rabbit mAb (AAA28540) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat liver using Ceruloplasmin Rabbit mAb (AAA28540) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using Ceruloplasmin Rabbit mAb (AAA28540) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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Ceruloplasmin, Monoclonal Antibody (Cat# AAA28540)

Full Name

Ceruloplasmin Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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Application Data (Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data (Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488)

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MHC CLASS II RT1B, Monoclonal Antibody (Cat# AAA12157)

Full Name

MOUSE ANTI RAT MHC CLASS II RT1B:FITC

Applications

Flow cytometry (FC/FACS)

Pricing

$515/0.1 mg $990/2x0.1 mg $1,440/3x0.1 mg $1,865/4x0.1 mg $2,065/5x0.1 mg

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WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)

WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

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Hsp90, Monoclonal Antibody (Cat# AAA17794)

Full Name

Hsp90 Antibody: HRP

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine

Applications

Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

Pricing

$615/0.2 mg $1,185/2x0.2 mg $1,725/3x0.2 mg $2,235/4x0.2 mg $2,435/5x0.2 mg

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WB (Western Blot) (Western blot analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SNX1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNX1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SNX1 expression in transfected 293T cell line by SNX1 monoclonal antibody. Lane 1: SNX1 transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SNX1 monoclonal antibody, Western Blot analysis of SNX1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SNX1, Monoclonal Antibody (Cat# AAA24962)

Full Name

SNX1 (Sorting Nexin-1, MGC8664) (Biotin)

Gene Names

SNX1; VPS5; HsT17379

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])

WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)

WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)

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NODAL, Monoclonal Antibody (Cat# AAA25474)

Full Name

NODAL (Nodal Homolog, MGC138230) (HRP)

Gene Names

NODAL; HTX5

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.Lane 1: GAPDH transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in Raw 264.7.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in PC-12.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in NIH/3T3.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in HeLa.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431.)

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GAPDH, Monoclonal Antibody (Cat# AAA25986)

Full Name

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase, G3PD, GAPD, MGC88685) (AP)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Applications

Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in Raw 264.7.)

WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10 Western Blot analysis of CDK4 expression in HeLa.)

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CDK4, Monoclonal Antibody (Cat# AAA26498)

Full Name

CDK4 (Cyclin-Dependent Kinase 4, CMM3, MGC14458, PSK-J3) (HRP)

Gene Names

CDK4; CMM3; PSK-J3

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Overlay histogram showing Raw264.7 cells stained with AAA27010 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10, 000 events was performed.)

IHC (Immunohistochemistry) (IHC image of AAA27010 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohistochemistry) (IHC image of AAA27010 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

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CD163, Monoclonal Recombinant Antibody (Cat# AAA27010)

Full Name

CD163 Antibody

Gene Names

CD163; M130; MM130; SCARI1

Reactivity

Human

Applications

ELISA, IHC, FC

Purity

Affinity-chromatography

Pricing

$275/0.05 mL $370/0.1 mL $710/2x0.1 mL $1,030/3x0.1 mL $1,330/4x0.1 mL $1,625/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

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MUC2, Monoclonal Antibody (Cat# AAA30338)

Full Name

MUC2 Antibody

Gene Names

MUC2; MLP; SMUC; MUC-2

Reactivity

Human, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12163)

Full Name

RAT ANTI MOUSE F4/80:Biotin

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow cytometry (FC/FACS)

Pricing

$780/0.5 mg $1,510/2x0.5 mg $2,200/3x0.5 mg $2,850/4x0.5 mg $3,265/5x0.5 mg

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WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in PC-12.)

WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged NME2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4 Western Blot analysis of NME2 expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

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NME2, Monoclonal Antibody (Cat# AAA26240)

Full Name

NME2 (Non-Metastatic Cells 2, Protein (NM23B) Expressed in, MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf) (Biotin)

Gene Names

NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: RPE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: AZIDE FREE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150:ALEXA 647)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150: Alexa Fluor® 488)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150)

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CD150, Monoclonal Antibody (Cat# AAA26752)

Full Name

CD150 (CD150 Antigen, Cdw150, 4933415F16, Estm51, Ipo 3, Ipo-3, OTTHUMP00000060252, Signaling Lymphocytic Activation Molecule, SLAM, Signaling Lymphocytic Activation Molecule Family Member 1, SLAMF1 Protein) (HRP)

Reactivity

Mouse

Applications

Flow Cytometry (FC/FACS)

Purity

Purified by Protein G Affinity Chromatography

Pricing

$960/0.1 mL $1,860/2x0.1 mL $2,710/3x0.1 mL $3,515/4x0.1 mL $4,080/5x0.1 mL

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Application Data (Overlay histogram showing SY5Y cells stained with AAA27008 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (10ug/1x10^6cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

IF (Immunofluorescence) (Immunofluorescent analysis of U87 cells using AAA27008 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of U251 cells using AAA27008 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of SY5Y cells using AAA27008 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of A549 cells using AAA27008 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney tissue using AAA27008 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human glioma using AAA27008 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain tissue using AAA27008 at dilution of 1:100)

WB (Western Blot) (Western BlotPositive WB detected in: Mouse brain tissue, Rat brain tissueAll lanes: GFAP antibody at 2.7ug/mlPredicted band size: 50, 51 kDaObserved band size: 50 kDa)

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GFAP, Monoclonal Antibody (Cat# AAA27008)

Full Name

GFAP Monoclonal Antibody

Gene Names

GFAP; ALXDRD

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

>95%, Protein G purified

Pricing

$275/0.05 mL $370/0.1 mL $710/2x0.1 mL $1,030/3x0.1 mL $1,330/4x0.1 mL $1,625/5x0.1 mL

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12161)

Full Name

RAT ANTI MOUSE F4/80:APC

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow cytometry (FC/FACS)

Pricing

$645/100 Tests $1,245/2x100 Tests $1,815/3x100 Tests $2,350/4x100 Tests $2,650/5x100 Tests

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WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)

WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

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Hsp90, Monoclonal Antibody (Cat# AAA17786)

Full Name

Hsp90 Antibody: FITC

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine

Applications

Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

Pricing

$625/0.2 mg $1,205/2x0.2 mg $1,755/3x0.2 mg $2,275/4x0.2 mg $2,480/5x0.2 mg

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WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in human colon.)

WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in HeLa.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with MAPK8 rabbit purified polyclonal 1:1200 and 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged SMAD3 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by. Lane 1: SMAD3 transfected lysate (48kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of SMAD3 expression in PC-12 using.)

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SMAD3, Monoclonal Antibody (Cat# AAA24954)

Full Name

SMAD3 (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, MGC60396) (Biotin)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PLXNA2 is 0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of PLXNA2 expression in transfected 293T cell line by PLXNA2 monoclonal antibody. Lane 1: PLXNA2 transfected lysate (Predicted MW: 18.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in NIH/3T3.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in Raw 264.7.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in HepG2)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (43.78kD).)

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PLXNA2, Monoclonal Antibody (Cat# AAA25210)

Full Name

PLXNA2 (Plexin A2, KIAA0463, OCT, PLXN2, Plexin-A2, Semaphorin Receptor OCT, FLJ11751, FLJ30634) (FITC)

Gene Names

PLXNA2; OCT; PLXN2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with C2399-07G2, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using C2399-07G2.)

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CD45, Monoclonal Antibody (Cat# AAA26746)

Full Name

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC)

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Equine, Human

Applications

Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

Purified by Protein A Affinity Chromatography

Pricing

$960/0.1 mL $1,860/2x0.1 mL $2,710/3x0.1 mL $3,515/4x0.1 mL $4,080/5x0.1 mL

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WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)

WB (Western Blot) (EIF4G1 monoclonal antibody, Western Blot analysis of EIF4G1 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged EIF4G1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF4G1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody. Lane 1: EIF4G1 transfected lysate (70.95kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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EIF4G1, Monoclonal Antibody (Cat# AAA24200)

Full Name

EIF4G1 (Eukaryotic Translation Initiation Factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220, EIF4G1, EIF4F, EIF4G, EIF4GI) (AP)

Gene Names

EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTN4 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody. Lane 1: ACTN4 transfected lysate (104.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in PC-12.)

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ACTN4, Monoclonal Antibody (Cat# AAA24712)

Full Name

ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (Biotin)

Gene Names

ACTN4; FSGS; FSGS1; ACTININ-4

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)

Application Data (Detection limit for recombinant GST tagged SSH3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1-10ug/ml])

WB (Western Blot) (Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody. Lane 1: SSH3 transfected lysate (73kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SSH3, Monoclonal Antibody (Cat# AAA24968)

Full Name

SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (Biotin)

Gene Names

SSH3; SSH3L

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SPP1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in NIH/3T3.)

WB (Western Blot) (SPP1 monoclonal antibody, Western Blot analysis of SPP1 expression in Hela NE.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in COLO 320 HSR.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in HeLa.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in human stomach.)

WB (Western Blot) (Western Blot detection against Immunogen (59.11kD).)

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Osteopontin, Monoclonal Antibody (Cat# AAA25480)

Full Name

Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) (HRP)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:RPE (MCA2872PE))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 488 (MCA2872A488))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 647 (MCA2872A647))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:FITC (MCA2872F)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872GA))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872T))

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CD49d, Monoclonal Antibody (Cat# AAA26760)

Full Name

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (HRP)

Reactivity

Rat

Applications

Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Purity

Purified by Protein G Affinity Chromatography

Pricing

$960/0.1 mL $1,860/2x0.1 mL $2,710/3x0.1 mL $3,515/4x0.1 mL $4,080/5x0.1 mL

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IP (Immunoprecipitation) (Tubulin was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate Lane C:0.5 mg Raw246.7 Whole Cell Lysate Lane D:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-Tubulin rabbit monoclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Tubulin rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size :54 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of Beta-Tubulin in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human Beta-Tubulin Chimera Mab (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human Beta-Tubulin in human lung cancer with Chimera Mab at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human Beta-Tubulin in human malignant melanoma with Chimera Mab at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining of human Beta-Tubulin in human breast carcinoma with Chimera Mab at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-Beta-Tubulin rabbit monoclonal antibody at 1:30000 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: A549 Whole Cell LysateLane E: Mouse brain tissue lysateLane F: Rat brain tissue lysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:50 kDaObserved band size:54 kDa)

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Beta-Tubulin, Monoclonal Recombinant Antibody (Cat# AAA27784)

Full Name

Beta-Tubulin Loading Control Antibody, Chimeric Rabbit MAb

Reactivity

Human

Applications

Western Blot (WB)(FAQ Protocol), Immunohistochemistry-Paraffin (IHC-P)(FAQ Protocol), Immunocytochemistry (ICC)/Immunofluorescence (IF)(FAQ Protocol), Immunoprecipitation (IP)(FAQ Protocol)

Purity

Protein A

Pricing

$185/0.02 mL $300/0.1 mL $575/2x0.1 mL $830/3x0.1 mL $1,070/4x0.1 mL $1,130/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1, 000 dilution.)

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Glucose 6 Phosphate Dehydrogenase, Monoclonal Antibody (Cat# AAA30344)

Full Name

Glucose 6 Phosphate Dehydrogenase Antibody

Gene Names

G6PD; G6PD1

Reactivity

Human

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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SDS-PAGE (SDS-PAGE Analysis Purified ATG5 Mouse Monoclonal Antibody (ATG5/2492). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot) (Western Blot Analysis of THP1 cell lysate and Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

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ATG5, Monoclonal Antibody (Cat# AAA23947)

Full Name

ATG5 (Autophagy Marker)

Gene Names

ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (69.01kD).)

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ADRM1, Monoclonal Antibody (Cat# AAA24715)

Full Name

ADRM1 (GP110, Proteasomal Ubiquitin Receptor ADRM1, 110kD Cell Membrane Glycoprotein, Adhesion-regulating Molecule 1, Proteasome Regulatory Particle Non-ATPase 13, Rpn13 Homolog, MGC29536) (Biotin)

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (77.26kD).)

IP (Immunoprecipitation) (Immunoprecipitation of STK38 transfected lysate using STK38 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK38 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK38 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue [antibody concentration 5ug/ml])

WB (Western Blot) (STK38 monoclonal antibody Western Blot analysis of STK38 expression in human kidney.)

WB (Western Blot) (Western Blot analysis of STK38 expression in transfected 293T cell line by STK38 monoclonal antibody Lane 1: STK38 transfected lysate (54.2kD). Lane 2: Non-transfected lysate.)

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STK38, Monoclonal Antibody (Cat# AAA24971)

Full Name

STK38 (Serine/Threonine-protein Kinase 38, NDR1 Protein Kinase, Nuclear Dbf2-related Kinase 1, NDR1) (Biotin)

Gene Names

STK38; NDR; NDR1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (RNF12 monoclonal antibody, Western Blot analysis of RNF12 expression in HeLa NE.)

Application Data (Detection limit for recombinant GST tagged RNF12 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RNF12 transfected lysate using RNF12 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with RNF12 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30ug/ml].)

WB (Western Blot) (Western Blot analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody. Lane 1: RNF12 transfected lysate (68.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (35.24kD).)

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RLIM, Monoclonal Antibody (Cat# AAA25227)

Full Name

RLIM (RING Finger LIM Domain-binding Protein, R-LIM, E3 Ubiquitin-protein Ligase RLIM, LIM Domain-interacting RING Finger Protein, RING Finger Protein 12, RNF12, Renal Carcinoma Antigen NY-REN-43) (FITC)

Gene Names

RLIM; MRX61; RNF12; TOKAS; NY-REN-43

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

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Leptin, Monoclonal Antibody (Cat# AAA30347)

Full Name

Leptin Receptor Antibody

Gene Names

LEPR; OBR; OB-R; CD295; LEP-R; LEPRD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

FCM (Flow Cytometry) (Fluorescence multiplex immunohistochemical analysis of Mouse)

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Glial Fibrillary Acidic Protein (GFAP), Monoclonal Antibody (Cat# AAA30859)

Full Name

Glial Fibrillary Acidic Protein (GFAP)(PTR2201/470) Antibody

Reactivity

Human, Mouse, Rat, Monkey, Bovine

Applications

Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)

Purity

Protein G

Pricing

$220/0.02 mL $285/0.05 mL $345/0.1 mL $560/0.2 mL $1,080/2x0.2 mL $1,570/3x0.2 mL $2,035/4x0.2 mL $2,245/5x0.2 mL

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Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488 (AAA11916A488))

Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647 (AAA11916A647))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)

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CD59, Monoclonal Antibody (Cat# AAA11916)

Full Name

MOUSE ANTI HUMAN CD59

Gene Names

CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

Applications

Immunohistology Frozen, ELISA (EIA), Immunoelectron Microscopy (EM), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*, Western Blot (WB)*

Pricing

$560/0.2 mg $1,080/2x0.2 mg $1,570/3x0.2 mg $2,035/4x0.2 mg $2,275/5x0.2 mg

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12172)

Full Name

RAT ANTI MOUSE F4/80:RPE

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

$285/25 Tests $545/2x25 Tests $785/3x25 Tests $1,015/4x25 Tests $1,030/5x25 Tests

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WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)

Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)

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CCT7, Monoclonal Antibody (Cat# AAA24454)

Full Name

CCT7 (T-complex Protein 1 Subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting Protein, CCTH, NIP7-1) APC

Gene Names

CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPL36A on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (RPL36A monoclonal antibody (M01), clone 5F8. Western Blot analysis of RPL36A expression in Raw 264.7.)

WB (Western Blot) (RPL36A monoclonal antibody (M01), clone 5F8. Western Blot analysis of RPL36A expression in PC-12.)

WB (Western Blot) (RPL36A monoclonal antibody (M01), clone 5F8. Western Blot analysis of RPL36A expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RPL36A is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (RPL36A monoclonal antibody (M01), clone 5F8. Western Blot analysis of RPL36A expression in HeLa.)

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RPL36A, Monoclonal Antibody (Cat# AAA25990)

Full Name

RPL36A (Ribosomal Protein L36a, L44L, MGC72020, MIG6, RPL44) (AP)

Gene Names

RPL36A; L36A; L44L; MIG6; RPL44

Applications

Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.