IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP6V1F protein using ATP6V1F antibody)
Mouse ATP6V1F Monoclonal Antibody | anti-ATP6V1F antibody
ATP6V1F antibody
Gene Names
ATP6V1F; VATF; Vma7; ATP6S14
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
ATP6V1F antibody was purified by affinity chromatography.
Synonyms
ATP6V1F, Antibody; ATP6V1F antibody; Monoclonal ATP6V1F; Anti-ATP6V1F; ATP6S14; VATF; Vma7; Vacuolar proton pump subunit F; V-ATPase subunit F; MGC117321; Adenosinetriphosphatase; Vacuolar ATP synthase subunit F; ATP6V1F; ATPV1F 6; ATPV1F-6; anti-ATP6V1F antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
5F10
Purity/Purification
ATP6V1F antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
119
Applicable Applications for anti-ATP6V1F antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification ofeukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists ofthree A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATPcatalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d.
Cross-Reactivity
Human, Dog, Rat, Monkey
Immunogen
ATP6V1F antibody was raised in mouse using a full length recombinant protein of human ATP6V1F (NP_004222) produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP6V1F protein using ATP6V1F antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ATP6V1F protein using ATP6V1F antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP6V1F protein (Right) or empty vector (Left) detected with ATP6V1F antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ATP6V1F protein (Right) or empty vector (Left) detected with ATP6V1F antibody)
Related Product Information for anti-ATP6V1F antibody
Mouse monoclonal ATP6V1F antibody
Product Categories/Family for anti-ATP6V1F antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,402 Da
NCBI Official Full Name
V-type proton ATPase subunit F isoform 1
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
NCBI Official Symbol
ATP6V1F
NCBI Official Synonym Symbols
VATF; Vma7; ATP6S14
NCBI Protein Information
V-type proton ATPase subunit F
UniProt Protein Name
V-type proton ATPase subunit F
UniProt Gene Name
ATP6V1F
UniProt Synonym Gene Names
ATP6S14; VATF; V-ATPase subunit F
UniProt Entry Name
VATF_HUMAN
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Product Notes
The ATP6V1F atp6v1f (Catalog #AAA106315) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ATP6V1F can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ATP6V1F atp6v1f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP6V1F, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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