WB (Western Blot)
(Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A, D), untreated human 4E-BP1 transfected (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A, B, C) and 4E-BP1 (D, E, F).)
Mouse 4E-BP1/2/3 Monoclonal Antibody | anti-4E-BP1/2/3 antibody
4E-BP1/2/3 (Phospho-Thr 45) Antibody
Gene Names
ABCA1; TGD; ABC1; CERP; ABC-1; HDLDT1
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
ProA affinity purified
Synonyms
4E-BP1/2/3, Antibody; 4E-BP1/2/3 (Phospho-Thr 45) Antibody; 4E-BP1 antibody; 4EBP1 antibody; 4EBP1_HUMAN antibody; BP 1 antibody; eIF4E binding protein 1 antibody; eIF4E-binding protein 1 antibody; Eif4ebp1 antibody; Eukaryotic translation initiation factor 4E-binding protein 1 antibody; PHAS-I antibody; PHASI antibody; Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody; anti-4E-BP1/2/3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
3G2
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-4E-BP1/2/3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
WB (Western Blot)
(Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A, D), untreated human 4E-BP1 transfected (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A, B, C) and 4E-BP1 (D, E, F).)
WB (Western Blot)
(Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A, D), untreated human 4E-BP1 transfected (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A, B, C) and 4E-BP1 (D, E, F).)
WB (Western Blot)
(Western blot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.)
WB (Western Blot)
(Western blot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.)
Related Product Information for anti-4E-BP1/2/3 antibody
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.
Product Categories/Family for anti-4E-BP1/2/3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
254,302 Da
NCBI Official Full Name
ATP-binding cassette sub-family A member 1
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 1
NCBI Official Symbol
ABCA1
NCBI Official Synonym Symbols
TGD; ABC1; CERP; ABC-1; HDLDT1
NCBI Protein Information
ATP-binding cassette sub-family A member 1; membrane-bound; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein
UniProt Protein Name
ATP-binding cassette sub-family A member 1
UniProt Gene Name
ABCA1
UniProt Synonym Gene Names
ABC1; CERP; ABC-1
UniProt Entry Name
ABCA1_HUMAN
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Product Notes
The 4E-BP1/2/3 abca1 (Catalog #AAA310988) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1/2/3 (Phospho-Thr 45) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1/2/3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the 4E-BP1/2/3 abca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1/2/3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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