Mouse anti-Human A20/TNFAIP3 Monoclonal Antibody | anti-TNFAIP3 antibody

A20/TNFAIP3 (Marker of Tamoxifen Resistance)

Cat. Num. AAA215364

• Gene Names: TNFAIP3; A20; AISBL; OTUD7C; TNFA1P2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: A20/TNFAIP3, Antibody; A20/TNFAIP3 (Marker of Tamoxifen Resistance); A20; Putative DNA-binding protein A20; TNF alpha-induced protein 3 (TNFAIP3); Tumor Necrosis Factor alpha-Induced Protein 3(TNFAIP3); Zinc finger protein A20; anti-TNFAIP3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: TNFAIP3/2813
• Specificity: A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. High A20 expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 790

• Applicable Applications for anti-TNFAIP3 antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant fragment (around aa 241-356) of Human TNFAIP3 protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic, Cell Surfaceand Nuclear
• Hu-chromosome Location: 6q23
• Positive Control: Hodgkin's Lymphoma or Hepatocellular carcinoma (HCC).
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human breast carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with TNFAIP3 Mouse Monoclonal Antibody (TNFAIP3/2813).)

References

Schmitz R, et al. TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J Exp Med. 2009 May 11; 206(5):981-9. | J A Vendrell,et al. A20/TNFAIP3, a new estrogen-regulated gene that confers tamoxifen resistance in breast cancer cells. Oncogene,26:4656-4667; 2007.

NCBI and Uniprot Product Information

• NCBI GI #: 395393995
• NCBI GeneID: 7128
• NCBI Accession #: NP_001257436.1
• NCBI GenBank Nucleotide #: NP_001257436.1
• UniProt Accession #: P21580
• Molecular Weight: 90kDa
• NCBI Official Full Name: tumor necrosis factor alpha-induced protein 3
• NCBI Official Synonym Full Names: TNF alpha induced protein 3
• NCBI Official Symbol: TNFAIP3
• NCBI Official Synonym Symbols: A20; AISBL; OTUD7C; TNFA1P2
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 3
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 3
• UniProt Gene Name: TNFAIP3
• UniProt Synonym Gene Names: OTUD7C; TNF alpha-induced protein 3
• UniProt Entry Name: TNAP3_HUMAN