Rabbit TNFAIP3 Polyclonal Antibody | anti-TNFAIP3 antibody

TNFAIP3 Antibody

Cat. Num. AAA168294

• Gene Names: TNFAIP3; A20; OTUD7C; TNFA1P2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: TNFAIP3, Antibody; TNFAIP3 Antibody; A20; MGC104522; MGC138687; MGC138688; OTUD7C; TNFA1P2; anti-TNFAIP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 790

• Applicable Applications for anti-TNFAIP3 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human TNFAIP3
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-17856 / sc-32523 / sc-32524 / sc-32525 / sc-22834
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cell using TNFAIP3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human stomach cancer using TNFAIP3 antibody at dilution of 1:200 (400x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using TNFAIP3 antibody.)

Related Product Information for anti-TNFAIP3 antibody

A20, also referred to as TNF-alpha-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-kappaB (1, 2). It was first identified as a TNF-alpha inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs (3). Constitutive expression of A20 is observed in lymphoid tissues (4), but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-alpha (3, 5), IL-1 (3, 5), phorbol esters (6), and LPS (7). Expression of A20 can confer resistance to apoptosis and NF-kappaB activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members (8, 9), and interaction with the NF-kappaB inhibiting protein ABIN (10). Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions (11, 12). The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation (12).

NCBI and Uniprot Product Information

• NCBI GI #: 395393997
• NCBI GeneID: 7128
• NCBI Accession #: NP_001257437.1
• NCBI GenBank Nucleotide #: NM_001270508.1
• Molecular Weight: 89,614 Da
• NCBI Official Full Name: tumor necrosis factor alpha-induced protein 3
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 3
• NCBI Official Symbol: TNFAIP3
• NCBI Official Synonym Symbols: A20; OTUD7C; TNFA1P2
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 3; OTU domain-containing protein 7C; TNF alpha-induced protein 3; putative DNA-binding protein A20; tumor necrosis factor inducible protein A20; zinc finger protein A20
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 3
• UniProt Gene Name: TNFAIP3
• UniProt Synonym Gene Names: OTUD7C; TNF alpha-induced protein 3
• UniProt Entry Name: TNAP3_HUMAN