WB (Western Blot)
(The mouse tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: Mouse Lung Tissue lysate;Lane 2.: HeLa cell lysate;Lane 3.: A549 cell lysate;Lane 4.: A431 cell lysate;Lane 5.: Raji cell lysate;)
Mouse anti-Human Actin Monoclonal Antibody | anti-ACTA1 antibody
Actin
Gene Names
ACTA1; ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Synonyms
Actin, Antibody; Actin; ACTA; actin, alpha 1, skeletal muscle; alpha skeletal muscle actin; alpha skeletal muscle; alpha-actin-1; ASMA; CFTD; CFTDM; MPFD; NEM1; NEM2; NEM3; anti-ACTA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT2F5
Form/Format
Liquid; In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
377
Applicable Applications for anti-ACTA1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Recombinant human Actin (13-377aa) purified from E Coli.
Conjugation
Unconjugated
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
WB (Western Blot)
(The mouse tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: Mouse Lung Tissue lysate;Lane 2.: HeLa cell lysate;Lane 3.: A549 cell lysate;Lane 4.: A431 cell lysate;Lane 5.: Raji cell lysate;)
WB (Western Blot)
(The mouse tissue and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Actin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: Mouse Lung Tissue lysate;Lane 2.: HeLa cell lysate;Lane 3.: A549 cell lysate;Lane 4.: A431 cell lysate;Lane 5.: Raji cell lysate;)
ICC (Immunocytochemistry)
(ICC/IF analysis of Actin in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
ICC (Immunocytochemistry)
(ICC/IF analysis of Actin in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Related Product Information for anti-ACTA1 antibody
ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Product Categories/Family for anti-ACTA1 antibody
References
Moreno CAM, et al., (2017) Pediatr. Neurol. 75: 11-16. Loumaye A,et al., (2017) J Cachexia Sarcopenia Muscle 8 (5): 768-777.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
actin, alpha skeletal muscle
NCBI Official Synonym Full Names
actin alpha 1, skeletal muscle
NCBI Official Symbol
ACTA1
NCBI Official Synonym Symbols
ACTA; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; SHPM; CFTD1; CFTDM
NCBI Protein Information
actin, alpha skeletal muscle
UniProt Protein Name
Actin, alpha skeletal muscle
UniProt Gene Name
ACTA1
UniProt Synonym Gene Names
ACTA
UniProt Entry Name
ACTS_HUMAN
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Product Notes
The ACTA1 acta1 (Catalog #AAA48281) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Actin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ACTA1 acta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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