Mouse Actin Monoclonal Antibody | anti-ACTB antibody

Actin (C-terminal region) Antibody

Cat. Num. AAA71559

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Rat, Mouse, Chicken
• Applications: Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot
• Synonyms: Actin, Antibody; Actin (C-terminal region) Antibody; Actin (C-terminal region); Actin M202; anti-ACTB antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse, Chicken
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: M202
• Specificity: This antibody detects a 42kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.
• Form/Format: Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-ACTB antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: Clone (M202) was generated from a sequence corresponding to amino acids in the C-terminal region of human beta-actin*. This human actin sequence is highly conserved in most eukaryotic actin isoforms.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

IHC (Immunohiostchemistry)

(Formalin fixed, citric acid treated parafin sections of E18 mouse skeletal muscle. Sections were probed with anti-Actin (AM2021) then anti-Mouse:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).)

WB (Western Blot)

(Western blot analysis of mouse C2C12 cells probed with mouse monoclonal anti-Actin (C-terminal region) antibody at 1:1000 (lane 1), 1:2000 (lane 2), or 1:4000 (lane 3).)

Related Product Information for anti-ACTB antibody

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four alpha isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (beta and gamma). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References

Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.
Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
Baba, T. et al. (2003) J. Immunol. 170: 3762.
Jungbluth, A. et al. (1995) FEBS Let. 375:87.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.3
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 375
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• UniProt Protein Name: Actin, cytoplasmic 1
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN