Application Data
Mouse anti-Mouse AHI-1 Monoclonal Antibody | anti-AHI-1 antibody
Anti-AHI-1 Antibody
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
AHI-1, Antibody; Anti-AHI-1 Antibody; Anti-C-mAhi-1 M5 Antibody; anti-AHI-1 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
It only specifically reacts with murine Ahi-1.
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, pH 7.4 with 0.02% sodium azide.
Concentration
100ug/100ul (varies by lot)
Sequence Length
10
Applicable Applications for anti-AHI-1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
C-mAhi-1 M5 reacts with an epitope in the c-terminal region of mouse Ahi-1 protein. A recombinant mouse Ahi-1 GST fusion protein was used as immunogen. This antibody was purified from supernatant by affinity chromatography.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Application Data
Application Data
Application Data
Application Data
Related Product Information for anti-AHI-1 antibody
Abelson helper integration site-1 (Ahi-1) is a novel oncogene identified based on its common activation in v-abl and myc-induced murine lymphomas. The murine Ahi-1 gene encodes a unique protein with multiple SH3 binding sites, an SH3 domain and multiple WD40-repeat domains. The conserved human homolog (AHI-1) contains an additional coiled-coil domain in its N-terminal region. Both murine and human Ahi-1/AHI-1 genes encode at least three isoforms and thus this gene is also subject to alternative splicing in normal cells. Expression of Ahi-1/AHI-1 is regulated at multiple stages of hematopoiesis in a highly conserved fashion in mouse and humans. It is highly elevated in certain human lymphoma and leukemia cells, including those from patients with cutaneous T-cell lymphomas (CTCL), BCR-ABL+ chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL). AHI-1 is also to be strongly expressed in embryonic hindbrain and forebrain, and AHI-1 may be required for both cerebellar and cortical development in humans. Defects in AHI-1 are the cause of Joubert syndrome, an autosomal recessive brain congenital malformation.
References
Chen, M et al. "Targeting primitive chronic myeloid leukemia cells by effective inhibition of a new AHI-1-BCR-ABL-JAK2 complex." J. Natl. Cancer Inst. 105:405-23 (2013). PubMed: 23446755.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors." Proteomics 12:2094-106 (2012). PubMed: 22623184.
Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI-1 in regulation of cellular resistance of BCR-ABL(+) chronic myeloid leukemia cells to tyrosine kinase inhibitors." Proteomics 12:2094-106 (2012). PubMed: 22623184.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Ahi-1 isoform I, partial
UniProt Protein Name
Ahi-1 isoform I
UniProt Gene Name
AHI-1
UniProt Entry Name
Q8NER0_HUMAN
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Product Notes
The AHI-1 ahi-1 (Catalog #AAA60782) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-AHI-1 Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AHI-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AHI-1 ahi-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHI-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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