WB (Western Blot)
(Anti-AK2 rabbit monoclonal antibody at 1:500 dilutionLane A: Mouse kidney tissue lysateLane B: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)
Rabbit anti-Human AK2 Monoclonal Antibody | anti-AK2 antibody
Recombinant Anti-AK2 Antibody, Rabbit Monoclonal
Gene Names
AK2; ADK2; AK 2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Protein A
Synonyms
AK2, Antibody; Recombinant Anti-AK2 Antibody, Rabbit Monoclonal; AK2/Adenylate kinase 2 Antibody, Rabbit MAb; adenylate kinase 2; Anti-ADK2 Antibody; Anti-AK2 Antibody; anti-AK2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
025
Specificity
Human AK2
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-AK2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human AK2 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human AK2 (rh AK2).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-AK2 rabbit monoclonal antibody at 1:500 dilutionLane A: Mouse kidney tissue lysateLane B: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)
WB (Western Blot)
(Anti-AK2 rabbit monoclonal antibody at 1:500 dilutionLane A: Mouse kidney tissue lysateLane B: HCT116 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human AK2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human AK2 in human kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human AK2 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human AK2 in human liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-AK2 antibody
Adenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals.
References
Lagresle-Peyrou C, et al. (2008) Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nature Genetics. 41: 106-11.Bruns GA, et al. (1977) Adenylate kinase 2, a mitochondrial enzyme. Biochem Genet. 15 (5-6): 477-86.Khler C, et al. (1999) Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis. FEBS Lett. 447 (1): 10-2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
232
NCBI Official Full Name
Adenylate kinase 2, mitochondrial
NCBI Official Synonym Full Names
adenylate kinase 2
NCBI Official Symbol
AK2
NCBI Official Synonym Symbols
ADK2; AK 2
NCBI Protein Information
adenylate kinase 2, mitochondrial; AK2; AK 2; ATP:AMP phosphotransferase; ATP-AMP transphosphorylase 2; adenylate monophosphate kinase; adenylate kinase isoenzyme 2, mitochondrial
UniProt Protein Name
Adenylate kinase 2, mitochondrial
UniProt Gene Name
AK2
UniProt Entry Name
KAD2_HUMAN
Similar Products
Product Notes
The AK2 ak2 (Catalog #AAA255640) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-AK2 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AK2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AK2 ak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.