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SDS-PAGE
(SDS-PAGE Analysis Purified Alkaline Phosphatase Recombinant Mouse Monoclonal (rALP/870). Confirmation of Purity and Integrity of Antibody.)
Mouse anti-Human Alkaline Phosphatase (Placental)/PLAP Monoclonal Antibody | anti-ALPP antibody
Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker) Mouse Monoclonal Antibody [Clone rALP/870]
Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Synonyms
Alkaline Phosphatase (Placental)/PLAP, Antibody; Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker) Mouse Monoclonal Antibody [Clone rALP/870]; Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker); Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP1; anti-ALPP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1, kappa
Clone Number
rALP/870
Form/Format
1.0mg/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Applicable Applications for anti-ALPP antibody
Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
Immunogen
Recombinant full-length human ALPP protein
Cellular Localization
Cytoplasmic, Cell surface
Positive Control
JEG-3 or HepG2 cells. Human placenta or seminoma.
Hu-chromosome Location
2q37.1
Unigene
284255
Preparation and Storage
Antibody without azide: store at -20 to -80°C
Non-hazardous. No MSDS required.
Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis Purified Alkaline Phosphatase Recombinant Mouse Monoclonal (rALP/870). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Alkaline Phosphatase Recombinant Mouse Monoclonal (rALP/870). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistchemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human colon. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistchemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human colon. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human heart. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human kidney. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry)
(IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using rALP/870 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.)
IHC (Immunohistochemistry-Formalin)
(IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using rALP/870 at 2ug/ml in PBS for 30min RT.)
IHC (Immunohistochemistry-Formalin)
(IHC analysis of formalin-fixed, paraffin-embedded human placenta stained using rALP/870 at 2ug/ml in PBS for 30min RT.)
Related Product Information for anti-ALPP antibody
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e. g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the identification of gestational trophoblastic disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70 kDa
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type; Regan isozyme; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme
UniProt Protein Name
Alkaline phosphatase, placental type
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
UniProt Entry Name
PPB1_HUMAN
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Product Notes
The ALPP alpp (Catalog #AAA23954) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Alkaline Phosphatase (Placental)/PLAP (Germ Cell Tumor Marker) Mouse Monoclonal Antibody [Clone rALP/870] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase (Placental)/PLAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Formalin (IHC-F). WB: 1-2ug/ml IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the ALPP alpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase (Placental)/PLAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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