anti-Human AMPK alpha 1 Monoclonal Antibody | anti-AMPKalpha1 antibody

Phospho-AMPK alpha 1 (Ser496) Rabbit mAb

Cat. Num. AAA314497

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity Purified
• Synonyms: AMPK alpha 1, Antibody; Phospho-AMPK alpha 1 (Ser496) Rabbit mAb; AMPK; AMPKa1; anti-AMPKalpha1 antibody

• Host: Recombinant Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: S01-3B7
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-AMPKalpha1 antibody: IF (Immunofluorescence), WB (Western Blot)
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser496 of human AMPK alpha 1
• Conjugation: Unconjugated
• Modification: Phosphorylated
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.

ICC (Immunocytochemistry)

(Immunocytochemistry of AMPK alpha 1 (phospho-Ser496)(green) in hela using AMPK alpha 1 (phospho-Ser496) Rabbit mAb at dilution 1/50, and DAPI(blue))

WB (Western Blot)

(Western blot detection of AMPK alpha 1 (phospho-Ser496) in Ramos cell lysates using AMPK alpha 1 (phospho-Ser496) Rabbit mAb(1:1000 diluted).Predicted band size:64kDa.Observed band size:64kDa.)

Related Product Information for anti-AMPKalpha1 antibody

Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357).In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation (PubMed:17307971, PubMed:17712357).AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators (PubMed:17307971, PubMed:17712357).Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively (By similarity).Promotes lipolysis of lipid droplets by mediating phosphorylation of isoform 1 of CHKA (CHKalpha2)(PubMed:34077757).

Product Categories/Family for anti-AMPKalpha1 antibody

Primary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 27469582
• NCBI GeneID: 5562
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 64,009 Da
• NCBI Official Full Name: PRKAA1 protein
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 1 catalytic subunit
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN