Rabbit anti-Human AMPKalpha1 Polyclonal Antibody | anti-AMPKalpha1 antibody

AMPKalpha1 (Phospho-Ser487)Antibody

Cat. Num. AAA305016

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: AMPKalpha1, Antibody; AMPKalpha1 (Phospho-Ser487)Antibody; AMPKa1 (Phospho-Ser487)Antibody; AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1; anti-AMPKalpha1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of AMPKa1 only when phosphorylated at serine 487.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 559

• Applicable Applications for anti-AMPKalpha1 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.
• Modification: Phospho-Ser487
• Target Name: AMPKa1
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

WB (Western Blot)

(Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKalpha1 (Phospho-Ser487) Antibody.)

IF (Immunofluorescence)

(Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1(Phospho-Ser487)Antibody.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Phospho-Ser487)Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot)

(Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKa1(Phospho-Ser487)Antibody.)

Related Product Information for anti-AMPKalpha1 antibody

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Product Categories/Family for anti-AMPKalpha1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 94557301
• NCBI GeneID: 5562
• NCBI Accession #: NP_006242.5
• NCBI GenBank Nucleotide #: NM_006251.5
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 65,523 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 1 catalytic subunit
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN