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IF (Immunofluorescence)
(ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAA11729 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human APP Monoclonal Antibody | anti-APP antibody
APP antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
APP, Antibody; APP antibody; amyloid beta A4 protein precursor; isoform; amyloid beta (A4) precursor protein; Alzheimer disease; peptidase nexin-II; anti-APP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
J4H9
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.
Concentration
1mg/ml (determined by BCA assay) (varies by lot)
Sequence Length
770
Applicable Applications for anti-APP antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
Recombinant human APP (18-289aa) purified from E. coli
Preparation and Storage
Can be stored at 2°C to 8°C.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAA11729 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAA11729 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
FCM (Flow Cytometry)
(Flow cytometry analysis of APP/Protease Nexin II in 293T cells. The cell was stained with AAA11729 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM (Flow Cytometry)
(Flow cytometry analysis of APP/Protease Nexin II in 293T cells. The cell was stained with AAA11729 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot)
(The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain tissue lysate.)
WB (Western Blot)
(The tissue lysate(40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain tissue lysate.)
WB (Western Blot)
(Tissue lysates of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(Tissue lysates of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human APP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-APP antibody
Amyloid precursor protein (APP) is the precursor molecule whose proteolysis generates amyloid beta (ABeta), a 39- to 42- amino acid peptide and this amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer’s diseases patients. APP is an integral membrane protein that is phosphorylated in the cytoplasmic and extracellular domains. It has been reported that cell-surface APP plays a role in neurite extension of primary cultured hippocampal neurons. The large extracellular domain of APP is also reported to bind extracellular matrix molecules such as heparin, laminin, and collagen, which can mediate cell adhesion and neurite outgrowth. Abnormal regulation of the metabolism of APP may contribute to the deposition of plaques.
Product Categories/Family for anti-APP antibody
References
Suzuki, T. et al., (1994) EMBO J. 13, 1114-1122; Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
amyloid-beta A4 protein isoform a
NCBI Official Synonym Full Names
amyloid beta precursor protein
NCBI Official Symbol
APP
NCBI Official Synonym Symbols
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
NCBI Protein Information
amyloid-beta A4 protein
UniProt Protein Name
Amyloid-beta A4 protein
UniProt Gene Name
APP
UniProt Synonym Gene Names
A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; Beta-CTF; Abeta42; Abeta40; Alpha-CTF; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
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Product Notes
The APP app (Catalog #AAA11729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The APP antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the APP app for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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