IHC (Immunohistochemisry)
(APP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human APP Polyclonal Antibody | anti-APP antibody
APP Antibody (Center)
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
APP, Antibody; APP Antibody (Center); Amyloid beta A4 protein; ABPP; APPI; APP; Alzheimer disease amyloid protein; Cerebral vascular amyloid peptide; CVAP; PreA4; Protease nexin-II; PN-II; N-APP; Soluble APP-alpha; S-APP-alpha; Soluble APP-beta; S-APP-beta; C99; Beta-amyloid protein 42; Beta-APP42; Beta-amyloid protein 40; Beta-APP40; C83; P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59; Amyloid intracellular domain 59; AICD-59; AID(59); Gamma-CTF(59); Gamma-secretase C-terminal fragment 57; Amyloid intracellular domain 57; AICD-57; AID(57); Gamma-CTF(57); Gamma-secretase C-terminal fragment 50; Amyloid intracellular domain 50; AICD-50; AID(50); Gamma-CTF(50); C31; A4; AD1; anti-APP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This APP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-362 amino acids from the Central region of human APP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
333-362
Sequence Length
746
Applicable Applications for anti-APP antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(APP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(APP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of APP Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of APP Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(APP Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the APP antibody detected the APP protein (arrow).)
WB (Western Blot)
(APP Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the APP antibody detected the APP protein (arrow).)
Related Product Information for anti-APP antibody
This gene encodes a cell surface receptor and
transmembrane precursor protein that is cleaved by secretases to
form a number of peptides. Some of these peptides are secreted and
can bind to the acetyltransferase complex APBB1/TIP60 to promote
transcriptional activation, while others form the protein basis of
the amyloid plaques found in the brains of patients with Alzheimer
disease. Mutations in this gene have been implicated in autosomal
dominant Alzheimer disease and cerebroarterial amyloidosis
(cerebral amyloid angiopathy). Multiple transcript variants
encoding several different isoforms have been found for this gene.
transmembrane precursor protein that is cleaved by secretases to
form a number of peptides. Some of these peptides are secreted and
can bind to the acetyltransferase complex APBB1/TIP60 to promote
transcriptional activation, while others form the protein basis of
the amyloid plaques found in the brains of patients with Alzheimer
disease. Mutations in this gene have been implicated in autosomal
dominant Alzheimer disease and cerebroarterial amyloidosis
(cerebral amyloid angiopathy). Multiple transcript variants
encoding several different isoforms have been found for this gene.
Product Categories/Family for anti-APP antibody
References
Dahse, K., et al. J. Mol. Biol. 403(4):643-659(2010)
Hare, J. Biochem. Biophys. Res. Commun. 401(2):219-224(2010)
Vossel, K.A., et al. Science 330 (6001), 198 (2010) :
Singh, T.D., et al. FEBS Lett. 584(18):3936-3942(2010)
Crews, L., et al. J. Neurosci. 30(37):12252-12262(2010)
Hare, J. Biochem. Biophys. Res. Commun. 401(2):219-224(2010)
Vossel, K.A., et al. Science 330 (6001), 198 (2010) :
Singh, T.D., et al. FEBS Lett. 584(18):3936-3942(2010)
Crews, L., et al. J. Neurosci. 30(37):12252-12262(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86943
NCBI Official Full Name
amyloid beta A4 protein isoform a
NCBI Official Synonym Full Names
amyloid beta (A4) precursor protein
NCBI Official Symbol
APP
NCBI Official Synonym Symbols
AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
NCBI Protein Information
amyloid beta A4 protein
UniProt Protein Name
Amyloid beta A4 protein
UniProt Gene Name
APP
UniProt Synonym Gene Names
A4; AD1; APP; CVAP; PN-II; S-APP-alpha; S-APP-beta; AICD-59; AID(59); AICD-57; AID(57); AICD-50; AID(50)
UniProt Entry Name
A4_HUMAN
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Product Notes
The APP app (Catalog #AAA284844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 333-362. The APP Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the APP app for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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