Mouse ARF1 Monoclonal Antibody | anti-ARF1 antibody

ARF1 (Golgi Apparatus Marker)

Cat. Num. AAA23921

• Gene Names: ARF1; PVNH8
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: ARF1, Antibody; ARF1 (Golgi Apparatus Marker); ADP-ribosylation factor 1; ARF1; anti-ARF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: ARF1/2117
• Specificity: The ADP-ribosylation factor (ARF) family comprises a group of structurally and functionally conserved proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. The ARF family is divided functionally into the ARF and the ARF-like proteins. ARF's share more than 60% sequence identity, appear to be ubiquitous in eukaryotes, and are highly conserved evolutionarily. ARF is involved in intracellular protein traffic to and within the Golgi complex. ARF has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 181

• Applicable Applications for anti-ARF1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa cells. Kidney, Liver, Brain or Breast.
• Immunogen: Recombinant full-length human ARF1 protein
• Cellular Localization: Cytoplasmic (Golgi Apparatus)
• Hu-chromosome Location: 1q42.13
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified ARF1 Mouse Monoclonal Antibody (ARF1/2117). Confirmation of Integrity and Purity of the Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Rat Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Breast stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Brain stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Liver stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Kidney stained with ARF1 Mouse Monoclonal Antibody (ARF1/2117).)

References

1. Puxeddu et al. 2009. Proc Natl Acad Sci U S A. 106(15):6158-63. 2. Austin et al. 2000. J Biol Chem. 275(29):21862-9.

NCBI and Uniprot Product Information

• NCBI GI #: 51316985
• NCBI GeneID: 375
• UniProt Accession #: P84077
• Molecular Weight: 20kDa
• NCBI Official Full Name: ADP-ribosylation factor 1
• NCBI Official Synonym Full Names: ADP ribosylation factor 1
• NCBI Official Symbol: ARF1
• NCBI Official Synonym Symbols: PVNH8
• NCBI Protein Information: ADP-ribosylation factor 1
• UniProt Protein Name: ADP-ribosylation factor 1
• UniProt Gene Name: ARF1
• UniProt Entry Name: ARF1_HUMAN