ATM recombinant antibody

ATM Antibody

Cat. Num. AAA235602

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity-Chromatography
• Synonyms: ATM, Recombinant Antibody; ATM Antibody; Serine-protein kinase ATM; Ataxia telangiectasia mutated; A-T mutated; ATM; ATM recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity-Chromatography
• Form/Format: Liquid; In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 3056

• Applicable Applications for ATM recombinant antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen Species: Human
• Immunogen: A synthesized peptide derived from human ATM
• Conjugate: Non-Conjugated
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating ATM in PC3 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA618770A0HU in PC3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA618770A0HU (3ug) + PC3 whole cell lysate (500ug)Lane 3: PC3 whole cell lysate (20ug))

IHC (Immunohistochemisry)

(IHC image of CSB-RA618770A0HU diluted at 1:205 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

IHC (Immunohiostchemistry)

(IHC image of CSB-RA618770A0HU diluted at 1:205 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysateAll lanes: ATM antibody at 2.05ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 350 KDaObserved band size: 350 KDa)

Related Product Information for ATM recombinant antibody

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.

Product Categories/Family for ATM recombinant antibody

Recombinant Antibody; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 71902540
• NCBI GeneID: 472
• NCBI Accession #: NP_000042.3
• NCBI GenBank Nucleotide #: NM_000051.3
• UniProt Accession #: Q13315; O15429; Q12758; Q16551; Q93007; Q9NP02; Q9UCX7; B2RNX5
• Molecular Weight: 350,687 Da
• NCBI Official Full Name: serine-protein kinase ATM isoform a
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM
• UniProt Protein Name: Serine-protein kinase ATM
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated