ICC (Immunocytochemistry)
(Immunocytochemical labeling of ATM phosphorylation in calyculin A-treated A431 cells. The cells were labeled with rabbit polyclonal anti-ATM (Ser-794) (AP3631) antibody in the absence (Left) or presence (Right) of blocking peptide (AX3635). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)
Rabbit ATM Polyclonal Antibody | anti-ATM antibody
ATM (Ser-794), phospho-specific Antibody
Gene Names
ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
Reactivity
Human, Rat, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, ELISA, Western Blot
Synonyms
ATM, Antibody; ATM (Ser-794), phospho-specific Antibody; ATM (Ser-794), phospho-specific; ATM Ser 794; anti-ATM antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was affinity purified using phospho-ATM (Ser-794) peptide (without carrier). The antibody detects a 370kDa* band corresponding to ATM on SDS-PAGE immunoblots of calyculin A treated Jurkat, A431, HeLa, and rat PC12 cells. This reactivity is removed after lambda phosphatase treatment.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-ATM antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-ATM (Ser-794) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-794 in human ATM. This sequence is well conserved in rat and mouse ATM.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of ATM phosphorylation in calyculin A-treated A431 cells. The cells were labeled with rabbit polyclonal anti-ATM (Ser-794) (AP3631) antibody in the absence (Left) or presence (Right) of blocking peptide (AX3635). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of ATM phosphorylation in calyculin A-treated A431 cells. The cells were labeled with rabbit polyclonal anti-ATM (Ser-794) (AP3631) antibody in the absence (Left) or presence (Right) of blocking peptide (AX3635). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)
WB (Western Blot)
(Western blot of human A431 cells treated with Calyculin A (100nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).)
WB (Western Blot)
(Western blot of human A431 cells treated with Calyculin A (100nM) for 30 min. Blot lanes were untreated (lanes 1, 3, & 5) or treated with lambda phosphatase (lanes 2, 4, & 6) then probed with anti-ATM (Ser-794) (lanes 1 & 2), anti-ATM (C-Terminal) (lanes 3 & 4), or anti-ATM (Ser-1981) (lanes 5 & 6).)
Related Product Information for anti-ATM antibody
Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. Activation of ATM after DNA damage involves Cdk5 mediated phosphorylation of Ser-794 followed by autophosphorylation at Ser-1891. Active ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis and DNA repair. The Cdk5-ATM pathway regulates phosphorylation and function of the ATM targets p53 and H2AX in postmitotic neurons. Other known substrates of ATM include Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1. Thus, activation of Cdk5 by DNA damage may be an important initiator of ATM-dependent regulation of cell cycle checkpoints.
References
Tian, B. et al. (2009) Nat Cell Biol. 11:211.
Lee, J.H. & Paull, T.T. (2007) Oncogene 26:7741.
Shiloh, Y. (1997) Annu Rev Genet. 31:635.
Lee, J.H. & Paull, T.T. (2007) Oncogene 26:7741.
Shiloh, Y. (1997) Annu Rev Genet. 31:635.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
350,714 Da
NCBI Official Full Name
Ataxia telangiectasia mutated
NCBI Official Synonym Full Names
ataxia telangiectasia mutated
NCBI Official Symbol
ATM
NCBI Official Synonym Symbols
AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
NCBI Protein Information
serine-protein kinase ATM; AT mutated; A-T mutated; TEL1, telomere maintenance 1, homolog
UniProt Protein Name
Serine-protein kinase ATM
UniProt Gene Name
ATM
UniProt Synonym Gene Names
A-T mutated
UniProt Entry Name
ATM_HUMAN
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Product Notes
The ATM atm (Catalog #AAA71565) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATM (Ser-794), phospho-specific Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATM can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ATM atm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATM, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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