IF (Immunofluorescence)
(ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human B2M Monoclonal Antibody | anti-B2M antibody
B2M antibody
Gene Names
B2M; IMD43
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
B2M, Antibody; B2M antibody; Beta-2-microglobulin; CDABP0092; HDCMA22P; anti-B2M antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT101F10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
119
Applicable Applications for anti-B2M antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human B2M (21-119aa) purified from E. coli
Clone Note
Anti-human B2M mAb, clone AT101F10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human B2M protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human B2M antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : U937 cell lysate)
WB (Western Blot)
(The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human B2M antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : HeLa cell lysate Lane 2 : U937 cell lysate)
Related Product Information for anti-B2M antibody
Beta2 microglobulin, also known as B2M, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Increased production or destruction of these cells causes B2M levels in the blood to increase. This increase is seen in people with cancers involving white blood cells, but it is particularly meaningful in people newly identified with multiple myeloma. Multiple myeloma is a malignancy (cancer) of a certain kind of white blood cell, called a plasma cell. B2M Testing is done primarily when evaluating a person for certain kinds of cancer affecting white blood cells including chronic lymphocytic leukemia, non-Hodgkin's lymphoma, and multiple myeloma or kidney disease.
Product Categories/Family for anti-B2M antibody
References
Huang WC., et al (2010) J Biol Chem. 285 (11): 7947-56. ; Morabito A., et al. (2009) Hum Immunol. 70 (7): 492-5.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,715 Da
NCBI Official Full Name
beta-2-microglobulin
NCBI Official Synonym Full Names
beta-2-microglobulin
NCBI Official Symbol
B2M
NCBI Official Synonym Symbols
IMD43
NCBI Protein Information
beta-2-microglobulin
UniProt Protein Name
Beta-2-microglobulin
UniProt Gene Name
B2M
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Product Notes
The B2M b2m (Catalog #AAA48257) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The B2M antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B2M can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the B2M b2m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B2M, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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