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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human Bak Monoclonal Antibody | anti-Bak antibody
Bak Antibody
Gene Names
BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Bak, Antibody; Bak Antibody; Apoptosis regulator BAK antibody; BAK antibody; BAK like antibody; Bak NT antibody; BAK_HUMAN antibody; Bak1 antibody; Bcl 2 homologous antagonist/killer antibody; Bcl 2 like 7 protein antibody; Bcl-2 homologous antagonist/killer antibody; Bcl-2-like protein 7 antibody; BCL2 antagonist/killer 1 antibody; Bcl2 like 7 Protein antibody; Bcl2-L-7 antibody; BCL2L7 antibody; CDN1 antibody; Cell death inhibitor 1 antibody; MGC117255 antibody; MGC3887 antibody; NBak antibody; Pro apoptotic protein BAK antibody; anti-Bak antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SU32-07
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Bak antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NIH/3T3 cells with Bak antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Bak in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Bak on different lysates using anti-Bak antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Human skeletal muscle Lane 3: Ags)
WB (Western Blot)
(Western blot analysis of Bak on different lysates using anti-Bak antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Human skeletal muscle Lane 3: Ags)
Related Product Information for anti-Bak antibody
The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax, accelerate death under various conditions. One member of the Bcl-2 family, designated Bak, functions primarily to enhance apoptotic cell death following appropriate activating signals and counteracts the protection from apoptosis provided by Bcl-2. Expression of Bak is widespread in a broad range of cells, including various long-lived, terminally differentiated cell types, suggesting that its cell-death-inducing activity is broadly distributed and that the regulation of inhibitors of apoptosis may represent an important determinant of tissue-specific modulation of apoptosis.
Product Categories/Family for anti-Bak antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,409 Da
NCBI Official Full Name
bcl-2 homologous antagonist/killer
NCBI Official Synonym Full Names
BCL2-antagonist/killer 1
NCBI Official Symbol
BAK1
NCBI Official Synonym Symbols
BAK; CDN1; BCL2L7; BAK-LIKE
NCBI Protein Information
bcl-2 homologous antagonist/killer; bcl2-L-7; BCL2-like 7 protein; bcl-2-like protein 7; apoptosis regulator BAK; pro-apoptotic protein BAK
UniProt Protein Name
Bcl-2 homologous antagonist/killer
UniProt Gene Name
BAK1
UniProt Synonym Gene Names
BAK; BCL2L7; CDN1; Bcl2-L-7
UniProt Entry Name
BAK_HUMAN
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Product Notes
The Bak bak1 (Catalog #AAA312206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bak Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bak can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Bak bak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bak, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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