Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Bcl-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse Bcl-6 Monoclonal Antibody | anti-Bcl-6 antibody
Bcl-6 (Follicular Lymphoma Marker)
Gene Names
BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
Bcl-6, Antibody; Bcl-6 (Follicular Lymphoma Marker); B-cell lymphoma 5 protein; B-Cell Lymphoma 6 Protein; BCL5; BCL6; BCL6A; cys his2 zinc finger transcription factor; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3); Zinc finger and BTB domain-containing protein 27 (ZBTB27); Zinc Finger Protein 51 (ZNF51); zinc finger transcription factor BCL6S; anti-Bcl-6 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
rBCL6/1527
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19, 000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of settings: (1) In the differential small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
706
Applicable Applications for anti-Bcl-6 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant fragment of human bcl-6 protein (around aa 256-389)
Cellular Localization
Nuclear
Hu-chromosome Location
3q27.3
Positive Control
Raji or Ramos cells. Tonsil or Hodgkins lymphoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Bcl-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Bcl-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis of Purified BCL-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527).)
SDS-PAGE
(SDS-PAGE Analysis of Purified BCL-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with BCL-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with BCL-6 Mouse Recombinant Monoclonal Antibody (rBCL6/1527).)
References
Ree, H.J., et al. 2003. Hum. Pathol. 34: 610-616. Garcia JF, et al.2006.J. Histochem Cytochem.54:31.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95kDa
NCBI Official Full Name
B-cell lymphoma 6 protein isoform 1
NCBI Official Synonym Full Names
B cell CLL/lymphoma 6
NCBI Official Symbol
BCL6
NCBI Official Synonym Symbols
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
NCBI Protein Information
B-cell lymphoma 6 protein
UniProt Protein Name
B-cell lymphoma 6 protein
UniProt Gene Name
BCL6
UniProt Synonym Gene Names
BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
Similar Products
Product Notes
The Bcl-6 bcl6 (Catalog #AAA214504) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Bcl-6 (Follicular Lymphoma Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-6 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Bcl-6 bcl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.