FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Jurkat cells stained with AAA279127 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
BCL6 recombinant antibody
BCL6 Recombinant Monoclonal Antibody
Gene Names
BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Reactivity
Escherichia coli, Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Affinity purified
Synonyms
BCL6, Recombinant Antibody; BCL6 Recombinant Monoclonal Antibody; B-cell lymphoma 6 protein (BCL-6)(B-cell lymphoma 5 protein)(BCL-5)(Protein LAZ-3)(Zinc finger and BTB domain-containing protein 27)(Zinc finger protein 51); BCL6; BCL5 LAZ3 ZBTB27 ZNF51; BCL6 recombinant antibody
Host
HEK293F Cell
Reactivity
Escherichia coli, Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
6E3
Purity/Purification
Affinity purified
Form/Format
Liquid; PBS, 150mM NaCl, 0.02% sodium azide, 50% glycerol, pH 7.4.
Applicable Applications for BCL6 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Immunogen Species
Human
Immunogen
A synthesized peptide derived from Human BCL6
Conjugation
Non-conjugated
Research Area
Epigenetics and Nuclear Signaling; Cancer
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Jurkat cells stained with AAA279127 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Jurkat cells stained with AAA279127 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4?. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4?.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IHC (Immunohistochemistry)
(IHC image of AAA279127 diluted at 1:33 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.73% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA279127 diluted at 1:33 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.73% DAB.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78,846 Da
NCBI Official Full Name
B-cell lymphoma 6 protein isoform 1
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 6
NCBI Official Symbol
BCL6
NCBI Official Synonym Symbols
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
NCBI Protein Information
B-cell lymphoma 6 protein; BCL-5; BCL-6; protein LAZ-3; zinc finger protein 51; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; zinc finger transcription factor BCL6S; cys-his2 zinc finger transcription factor; zinc finger and BTB domain-
UniProt Protein Name
B-cell lymphoma 6 protein
UniProt Gene Name
BCL6
UniProt Synonym Gene Names
BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
UniProt Entry Name
BCL6_HUMAN
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Product Notes
The BCL6 bcl6 (Catalog #AAA279127) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The BCL6 Recombinant Monoclonal Antibody reacts with Escherichia coli, Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCL6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the BCL6 bcl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL6, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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