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WB (Western Blot)
(Detection of human Bcl6 by western blot. Samples: Whole cell lysate (5 ug) from Ramos, Daudi, Raji, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
BCL6 recombinant antibody
Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H]
Gene Names
BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
Bcl6, Recombinant Antibody; Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H]; BCL6A; ZNF51; zinc finger transcription factor BCL6S; zinc finger protein 51; zinc finger and BTB domain-containing protein 27; ZBTB27; protein LAZ-3; lymphoma-associated zinc finger gene on chromosome 3; B-cell lymphoma 5 protein; cys-his2 zinc finger transcription factor; BCL-6; BCL-5; BCL5; B-cell lymphoma 6 protein transcript; B-cell lymphoma 6 protein; LAZ3; B cell CLL/lymphoma 6; BCL6 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR143H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for BCL6 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 375 and 425
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Bcl6 by western blot. Samples: Whole cell lysate (5 ug) from Ramos, Daudi, Raji, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human Bcl6 by western blot. Samples: Whole cell lysate (5 ug) from Ramos, Daudi, Raji, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human Bcl6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1) used for IP at 6 ul per reaction. Bcl6 was also immunoprecipitated by rabbit anti-Bcl6 antibody BL-1481A-1F2. For blotting immunoprecipitated Bcl6, AAA213604 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Bcl6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1) used for IP at 6 ul per reaction. Bcl6 was also immunoprecipitated by rabbit anti-Bcl6 antibody BL-1481A-1F2. For blotting immunoprecipitated Bcl6, AAA213604 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemisry)
(Detection of human Bcl6 in FFPE tonsil by IHC. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human Bcl6 in FFPE tonsil by IHC. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Bcl6 in FFPE Raji cells by ICC. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Bcl6 in FFPE Raji cells by ICC. Antibody: Rabbit anti-Bcl6 recombinant monoclonal antibody (AAA213604 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl6 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-Bcl6 recombinant monoclonal (AAA213604) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl6 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-Bcl6 recombinant monoclonal (AAA213604) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for BCL6 recombinant antibody
Bcl6 is a zinc finger transcription factor and contains an N-terminal POZ domain. It acts as a sequence-specific repressor of transcription and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. Bcl6 can interact with a variety of POZ-containing proteins that function as transcription corepressors. Bcl6 is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL) and may be involved in the pathogenesis of DLCL. [taken from NCBI Entrez Gene (Gene ID: 604)].
Product Categories/Family for BCL6 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,367 Da
NCBI Official Full Name
Homo sapiens B-cell CLL/lymphoma 6 (BCL6), transcript variant 1, mRNA
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 6
NCBI Official Symbol
BCL6
NCBI Official Synonym Symbols
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
NCBI Protein Information
B-cell lymphoma 6 protein; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; cys-his2 zinc finger transcription factor; lymphoma-associated zinc finger gene on chromosome 3; protein LAZ-3; zinc finger and BTB domain-containing
UniProt Protein Name
B-cell lymphoma 6 protein
UniProt Gene Name
BCL6
UniProt Synonym Gene Names
BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5
UniProt Entry Name
BCL6_HUMAN
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Product Notes
The BCL6 bcl6 (Catalog #AAA213604) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Bcl6 Recombinant Monoclonal Antibody [BLR143H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl6 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BCL6 bcl6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl6, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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