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IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
Mouse beta-Tubulin Monoclonal Antibody | anti-TUBB3 antibody
Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)
Gene Names
TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Synonyms
beta-Tubulin, Antibody; Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6); TUBB3; TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; anti-TUBB3 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster
Clonality
Monoclonal
Isotype
Mouse IgG1
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Form/Format
Liquid in PBS, pH7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TUBB3 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes
WB: 1:10000
IHC-P: 1:400
IF: 1:400
IHC-P: 1:400
IF: 1:400
Tag
TUBB3
Observed Band (KD)
55
Immunogen
Synthetic Peptide
Preparation and Storage
Stable for one year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping||Gel pack with blue ice.
Shipping||Gel pack with blue ice.
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Fig.4. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
IF (Immunofluorescence)
(Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
IF (Immunofluorescence)
(Fig.3. Immunofluorescence analysis of mouse lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
IF (Immunofluorescence)
(Fig.2. Immunofluorescence analysis of human appendix tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
IF (Immunofluorescence)
(Fig.2. Immunofluorescence analysis of human appendix tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
WB (Western Blot)
(Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.)
WB (Western Blot)
(Fig.1. Western blot analysis of A549(1), rat brain (2), mouse brain (3), chicken lung (4) and rabbit testis(5), sheep muscle(6), diluted at 1:10000.)
Related Product Information for anti-TUBB3 antibody
Description: Featured Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6), specially designed for your immunoassay as internal control or location staining.
Background: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55kDa. Antibodies against beta-Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells, such as adipose tissue.
Background: Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are alpha-tubulin and beta-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55kDa. Antibodies against beta-Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells, such as adipose tissue.
Product Categories/Family for anti-TUBB3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,433 Da
NCBI Official Full Name
tubulin beta-3 chain isoform 2
NCBI Official Synonym Full Names
tubulin, beta 3 class III
NCBI Official Symbol
TUBB3
NCBI Official Synonym Symbols
CDCBM; TUBB4; beta-4; CFEOM3A
NCBI Protein Information
tubulin beta-3 chain; tubulin beta-III; tubulin beta-4 chain; class III beta-tubulin
UniProt Protein Name
Tubulin beta-3 chain
UniProt Gene Name
TUBB3
UniProt Synonym Gene Names
TUBB4
UniProt Entry Name
TBB3_HUMAN
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Product Notes
The TUBB3 tubb3 (Catalog #AAA31540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6) reacts with Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Tubulin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF). WB: 1:10000 IHC-P: 1:400 IF: 1:400. Researchers should empirically determine the suitability of the TUBB3 tubb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Tubulin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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