WB (Western Blot)
(Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
BRD4 recombinant antibody
Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5]
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
ICC-IF: 1:25-1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
ChIP-Seq: A previous lot has qualified for ChIP-Seq. 10-40 ul per 30 ug chromatin.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human BRD4 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HEK293T, Jurkat, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human BRD4 by western blot. Samples: Whole cell lysate (25 ug) from HeLa, HEK293T, Jurkat, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (AAA23786 lot 3) and a second antibody against a different epitope of BRD4 . For blotting immunoprecipitated BRD4, AAA23786 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786 lot 4) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (AAA23786 lot 3) and a second antibody against a different epitope of BRD4 . For blotting immunoprecipitated BRD4, AAA23786 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (AAA23786-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (AAA23786-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human BRD4 by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786) used at 1:250. Secondary: DyLight 550-conjugated goat anti-rabbit IgG cross-adsorbed antibody .)
ICC (Immunocytochemistry)
(Detection of human BRD4 by immunocytochemistry. Sample: Formaldehyde-fixed HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (AAA23786) used at 1:250. Secondary: DyLight 550-conjugated goat anti-rabbit IgG cross-adsorbed antibody .)
ICC (Immunocytochemistry)
(Detection of human BRD4 by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (AAA23786-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human BRD4 by immunocytochemistry. Sample: FFPE section of HeLa cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody (AAA23786-4). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (AAA23786) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (AAA23786) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
ChIP (Chromatin Immunoprecipitation)
(Localization of BRD4 Binding Sites by ChIP-sequencing. Chromatin from Mia PaCa-2 cells was immunoprecipitated with anti-BRD4 antibody AAA23786 and analyzed by DNA sequencing. The figure illustrates the peak distribution of BRD4 binding within a 250 Kb region of chromosome 7 as detected using anti-BRD4 antibody AAA23786. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
ChIP (Chromatin Immunoprecipitation)
(Localization of BRD4 Binding Sites by ChIP-sequencing. Chromatin from Mia PaCa-2 cells was immunoprecipitated with anti-BRD4 antibody AAA23786 and analyzed by DNA sequencing. The figure illustrates the peak distribution of BRD4 binding within a 250 Kb region of chromosome 7 as detected using anti-BRD4 antibody AAA23786. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
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Product Notes
The BRD4 brd4 (Catalog #AAA23786) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-149-2H5] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunocytochemistry-Immunofluorescence (ICC-IF), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. ICC-IF: 1:25-1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells. ChIP-Seq: A previous lot has qualified for ChIP-Seq. 10-40 ul per 30 ug chromatin. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the BRD4 brd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRD4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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