BRD4 recombinant antibody

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]

Cat. Num. AAA23787

• Gene Names: BRD4; CAP; MCAP; HUNK1; HUNKI
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: BRD4; Monoclonal Recombinant Antibody; Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]; HUNK1; Protein HUNK1; HUNKI; chromosome-associated protein; CAP; bromodomain-containing protein 4; MCAP; Bromodomain-containing protein 4; bromodomain containing 4; BRD4 recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BL-151-6F11
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for BRD4 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Immunocytochemistry-Immunofluorescence (ICC-IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 20 ul/mg lysate; IHC: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; IHC-IF: 1:25-1:250. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF: 1:25-1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 1312 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot)

(Detection of human BRD4 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (lot AAA23787-2). For blotting immunoprecipitated BRD4, AAA23787 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

IHC (Immunohistochemistry)

(Detection of human BRD4 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human BRD4 by immunocytochemistry. Sample: formaldehyde-fixed Hep-G2 cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

FCM (Flow Cytometry)

(Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for BRD4 recombinant antibody

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Product Categories/Family for BRD4 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 19718731
• NCBI GeneID: 23476
• NCBI Accession #: NP_490597.1; NP_055114.1; NP_065254.3
• NCBI GenBank Nucleotide #: NM_058243.2
• UniProt Accession #: O60885; O60433; Q4G0X8; Q86YS8; Q96PD3
• Molecular Weight: 155.8kDa
• NCBI Official Full Name: bromodomain-containing protein 4 isoform long
• NCBI Official Synonym Full Names: bromodomain containing 4
• NCBI Official Symbol: BRD4
• NCBI Official Synonym Symbols: CAP; MCAP; HUNK1; HUNKI
• NCBI Protein Information: bromodomain-containing protein 4
• UniProt Protein Name: Bromodomain-containing protein 4
• UniProt Gene Name: BRD4
• UniProt Synonym Gene Names: HUNK1
• UniProt Entry Name: BRD4_HUMAN