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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.)
Mouse c-Cbl Monoclonal Antibody | anti-c-Cbl antibody
Anti-c-Cbl
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Ascitic Fluid
Synonyms
c-Cbl, Antibody; Anti-c-Cbl; CBL; CBL2; NSLL; C-CBL; RNF55; anti-c-Cbl antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6C5-D2-C6
Purity/Purification
Ascitic Fluid
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Sequence Length
906
Applicable Applications for anti-c-Cbl antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry Paraffin (IHC-P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry
Application Notes
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC: 1:200-1:400
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC: 1:200-1:400
Immunogen
Purified recombinant fragment of human C-CBL expressed in E Coli.
Antibody Type
Primary Antibody
Conjugation
Unconjugated
Modification
Unmodification
Cross Reactivity
Human, Mouse, Rat
Subcellular Location
Cell membrane, Cytoplasm, Membrane
Preparation and Storage
Store at 4°C short term.
Store at -20°C long term.
Avoid freeze/thaw cycles
Store at -20°C long term.
Avoid freeze/thaw cycles
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL mouse mAb with DAB staining.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL mouse mAb with DAB staining.)
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).)
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells using C-CBL mouse mAb (blue) and negative control (red).)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue)
IF (Immunofluorescence)
(Immunofluorescence analysis of Hela cells using C-CBL mouse mAb (green). Blue)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using c-Cbl mouse mAb.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of Hela cell lysates using c-Cbl mouse mAb.)
WB (Western Blot)
(Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.)
WB (Western Blot)
(Western blot analysis using C-CBL mouse mAb against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.)
WB (Western Blot)
(Western blot detection of c-Cbl in C6, Jurkat, K562, 3T3 and Hela cell lysates using c-Cbl mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.)
WB (Western Blot)
(Western blot detection of c-Cbl in C6, Jurkat, K562, 3T3 and Hela cell lysates using c-Cbl mouse mAb (1:1000 diluted).Predicted band size:120KDa.Observed band size:120KDa.)
Related Product Information for anti-c-Cbl antibody
Swiss-Prot Acc.P22681. The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase CBL
NCBI Official Synonym Full Names
Cbl proto-oncogene
NCBI Official Symbol
CBL
NCBI Official Synonym Symbols
CBL2; NSLL; C-CBL; RNF55; FRA11B
NCBI Protein Information
E3 ubiquitin-protein ligase CBL
UniProt Protein Name
E3 ubiquitin-protein ligase CBL
UniProt Gene Name
CBL
UniProt Synonym Gene Names
CBL2; RNF55
Similar Products
Product Notes
The c-Cbl cbl (Catalog #AAA14105) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's c-Cbl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry Paraffin (IHC-P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry. WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000 FC: 1:200-1:400. Researchers should empirically determine the suitability of the c-Cbl cbl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Cbl, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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