Mouse anti-Human Calcineurin B homologous protein 2/HCC Antigen 520 Monoclonal Antibody | anti-CHP2 antibody

Calcineurin B homologous protein 2/HCC Antigen 520

Cat. Num. AAA215192

• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Calcineurin B homologous protein 2/HCC Antigen 520, Antibody; Calcineurin B homologous protein 2/HCC Antigen 520; calcineurin-like EF hand protein 2; calcineurin-like EF-hand protein 2; HCA520; hepatocellular carcinoma antigen gene 520; Hepatocellular carcinoma-associated antigen 520 homolog; anti-CHP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: CPTC-CHP2-1
• Specificity: CHP2 (calcineurin B homologous protein 2), also known as hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains 4 EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 196

• Applicable Applications for anti-CHP2 antibody: IF (Immunofluorescence), WB (Western Blot)
• Positive Control: MCF-7, PC-3, HT29, K562 or Jurkat cell lysates. Human appendix or testis.
• Immunogen: Recombinant human full-length CHP2 protein
• Cellular Localization: Nucleus. Cytoplasm.
• Hu-chromosome Location: 16p12.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence)

(Immunofluorescence Analysis of human MCF-7 cells labeling CHP2 with CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

WB (Western Blot)

(Western Blot Analysis of MCF-7, PC3 cell lysate using CHP2 Mouse Monoclonal Antibody (CPTC-CHP2-1).)

References

Ammar, YB. et al. The EMBO Journal, EMBO Press, 7 June 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 11545811
• NCBI GeneID: 63928
• NCBI Accession #: NP_071380.1
• NCBI GenBank Nucleotide #: NP_071380.1
• UniProt Accession #: O43745
• Molecular Weight: 22kDa
• NCBI Official Full Name: calcineurin B homologous protein 2
• NCBI Official Synonym Full Names: calcineurin like EF-hand protein 2
• NCBI Official Symbol: CHP2
• NCBI Protein Information: calcineurin B homologous protein 2
• UniProt Protein Name: Calcineurin B homologous protein 2
• UniProt Gene Name: CHP2
• UniProt Synonym Gene Names: HCA520
• UniProt Entry Name: CHP2_HUMAN