IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Calprotectin Monoclonal Antibody (CPT/1028))
Mouse anti-Human Calprotectin Monoclonal Antibody | anti-S100A9 antibody
Calprotectin (Macrophage Marker) Mouse Monoclonal Antibody
Gene Names
S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Calprotectin, Antibody; Calprotectin (Macrophage Marker) Mouse Monoclonal Antibody; S100-A9; MRP14; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B); anti-S100A9 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM, kappa
Clone Number
CPT/1028
Specificity
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain. Calprotectin comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Sequence Length
114
Applicable Applications for anti-S100A9 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasmic
Immunogen
Recombinant human Calprotectin protein
Hu-Chromosome Location
1q21
Positive Control
Tonsil, lymph node, or spleen
Preparation and Storage
Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Calprotectin Monoclonal Antibody (CPT/1028))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Calprotectin Monoclonal Antibody (CPT/1028))
References
Cross, S.S., et al. 2005. Expression of S100 proteins in normal human tissues and common cancers using tissue microarrays: S100A6, S100A8, S100A9 and S100A11 are all overexpressed in common cancers. Histopathology 46: 256-269
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14kDa
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9
NCBI Official Symbol
S100A9
NCBI Official Synonym Symbols
MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
NCBI Protein Information
protein S100-A9
UniProt Protein Name
Protein S100-A9
UniProt Gene Name
S100A9
UniProt Synonym Gene Names
CAGB; CFAG; MRP14; MRP-14; p14
UniProt Entry Name
S10A9_HUMAN
Similar Products
Product Notes
The S100A9 s100a9 (Catalog #AAA62787) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Calprotectin (Macrophage Marker) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Calprotectin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the S100A9 s100a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calprotectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.