Mouse Catenin, beta (p120) Monoclonal Antibody | anti-CTNNB1 antibody

Catenin, beta (p120)

Cat. Num. AAA215409

• Gene Names: CTNNB1; EVR7; CTNNB; MRD19; NEDSDV; armadillo
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: Catenin, beta (p120), Antibody; Catenin, beta (p120); Cadherin associated protein; beta 1 88kDa; Catenin beta-1; CATNB; CHBCAT; CTNNB1; anti-CTNNB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: rCTNNB1/1507
• Specificity: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 781

• Applicable Applications for anti-CTNNB1 antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Recombinant Human beta-Catenin (p120) protein fragment
• Cellular Localization: Cell Surfaceand Cytoplasmic
• Antibody Type: Recombinant Antibody
• Hu-chromosome Location: 3p22.1
• Positive Control: HeLa or MCF-7 cells. Liver tissue lysate.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA fixed HeLa cells using Beta-Catenin (p120) Recombinant Mouse Monoclonal Antibody (rCTNNB1/1507) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human tonsil stained with Catenin, beta Recombinant Mouse Monoclonal Antibody (rCTNNB1/1507).)

SDS-PAGE

(SDS-PAGE Analysis Purified p120 Recombinant Mouse Monoclonal Antibody (rCTNNB1/1507). Confirmation of Purity and Integrity of Antibody.)

References

Dabbs DJ et. al. Am J Surg Path. 2007;31:427-437. | Sarrio D et. al. Oncogene. 2004;23:3272-3283. | Mastracci TL et. al. Mod Path. 2005;18:741-751.

NCBI and Uniprot Product Information

• NCBI GI #: 148233338
• NCBI GeneID: 1499
• NCBI Accession #: NP_001091679.1
• NCBI GenBank Nucleotide #: NP_001091679.1
• UniProt Accession #: P35222
• Molecular Weight: 92kDa
• NCBI Official Full Name: catenin beta-1 isoform 1
• NCBI Official Synonym Full Names: catenin beta 1
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: EVR7; CTNNB; MRD19; NEDSDV; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB
• UniProt Entry Name: CTNB1_HUMAN