FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 . U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of E11/19 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Mouse anti-Human CCR5 Monoclonal Antibody | anti-CCR5 antibody
Anti-CCR5 (phosphoserine 337) [V14/2]
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified antibody. Protein A affinity purified
Synonyms
CCR5, Antibody; Anti-CCR5 (phosphoserine 337) [V14/2]; CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; p337; pS337; Ser-337; Ser337; anti-CCR5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2b, kappa
Clone Number
V14/2
Specificity
V14/2 antibody has been demonstrated to exclusively recognise CCR5 (CD195) phosphorylated at Ser 337.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CCR5 antibody
IF (Immunofluorescence), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
PMID
12403770
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 . U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of E11/19 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19 . U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody or the rabbit IgG1 version of E11/19 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 337) antibody V14/2 Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of V14/2 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 337) antibody V14/2 Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of V14/2 at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CCR5 antibody
Recombinant monoclonal antibody to CCR5 (phosphoserine 337). Recombinant Platform with variable regions (i.e. specificity) from the hybridoma V14/2.
This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
Product Categories/Family for anti-CCR5 antibody
References
Pollok-Koppet et al. Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies. J Biol Chem. 2003 Jan 24;278(4):2190-8. Epub 2002 Oct 27.
Note on publication: This article describes the generation and characterization of the V14/2 antibody.
Note on publication: This article describes the generation and characterization of the V14/2 antibody.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,524 Da
NCBI Official Full Name
C-C chemokine receptor type 5
NCBI Official Synonym Full Names
chemokine (C-C motif) receptor 5 (gene/pseudogene)
NCBI Official Symbol
CCR5
NCBI Official Synonym Symbols
CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
NCBI Protein Information
C-C chemokine receptor type 5; chemr13; HIV-1 fusion coreceptor; chemokine receptor CCR5; C-C motif chemokine receptor 5 A159A
UniProt Protein Name
C-C chemokine receptor type 5
UniProt Gene Name
CCR5
UniProt Synonym Gene Names
CMKBR5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5
UniProt Entry Name
CCR5_HUMAN
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Product Notes
The CCR5 ccr5 (Catalog #AAA72176) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CCR5 (phosphoserine 337) [V14/2] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CCR5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CCR5 ccr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCR5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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