ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse anti-Human CD155/PVR Monoclonal Antibody | anti-CD155/PVR antibody
CD155/PVR (Extracellular region) Antibody
Gene Names
PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
Reactivity
Human
Applications
Immunocytochemistry, Immunoprecipitation, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
CD155/PVR, Antibody; CD155/PVR (Extracellular region) Antibody; CD155; anti-CD155/PVR antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M048
Specificity
Clone M048 mouse monoclonal antibody detects a 75kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human A549, NCI-H466, NCI-H1299, MDA-MB-231 and A431 carcinomas. The antibody detects CD155 in the membranes and cytoplasm in NCI-H446 cells after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CD155/PVR antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Clone (M048) was generated from a recombinant protein that included the extracellular region of human CD155 protein.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CD155 in aldehyde fixed and NP-40 permeabilized human NCI-H446 small cell lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD155 (CM0481). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.)
WB (Western Blot)
(Western blot of human A549 lung carcinoma (lane 1), NCI-H446 small cell lung carcinoma (lane 2), NCI-H1299 lung carcinoma (lane 3), MDA-MB-231 breast carcinoma (lane 4), and A431 epidermoid carcinoma (lane 5). The blot was probed with mouse monoclonal anti-CD155 (CM0481) at 1:500.)
Related Product Information for anti-CD155/PVR antibody
CD155/Poliovirus receptor (PVR)/nectin-like 5 (Necl-5) is a transmembrane glycoprotein with extracellular immunoglobulin like domains, and an intracellular immunoreceptor tyrosine-based inhibitor motif (ITIM). CD155 was originally described as a mediator of poliovirus attachment to cells, but has also been implicated in adherens junction formation. CD155 binds nectin-3, and interacts with integrin alphavbeta3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells. CD155 is also a ligand for immunoreceptors that regulate tumor surveillance. CD155 binds DNAX-associated molecule 1 (DNAX-1), an activating receptor on natural killer cells and cytotoxic T-cells. Alternatively, CD155 may bind TIGIT immunoreceptor inducing an immunosuppressive and non-cytotoxic profile. In cancers, CD155 expression has been associated with unfavorable prognosis in colon cancer, breast cancer, lung adenocarcinoma, pancreatic cancer, melanoma, and glioblastoma. Cancer therapies have targeted CD155 interactions with TIGIT, and have used CD155 as a point of entry for recombinant oncolytic polioviruses.
References
Gao, J. (2017) Cancer Sci. 108(10):1934.
Molfetta, R. (2020) Int J Mol Sci. 21(3):922.
Molfetta, R. (2020) Int J Mol Sci. 21(3):922.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,303 Da
NCBI Official Full Name
poliovirus receptor isoform alpha
NCBI Official Synonym Full Names
poliovirus receptor
NCBI Official Symbol
PVR
NCBI Official Synonym Symbols
PVS; HVED; CD155; NECL5; TAGE4; Necl-5
NCBI Protein Information
poliovirus receptor; nectin-like protein 5
UniProt Protein Name
Poliovirus receptor
UniProt Gene Name
PVR
UniProt Synonym Gene Names
PVS; NECL-5
UniProt Entry Name
PVR_HUMAN
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Product Notes
The CD155/PVR pvr (Catalog #AAA71588) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD155/PVR (Extracellular region) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD155/PVR can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD155/PVR pvr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD155/PVR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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