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WB (Western Blot)
(Detection of human PVR/CD155 by western blot. Samples: Whole cell lysate (10 ug) from 786-O, K-562, OVCAR-4, Jurkat, KM12, NCI-H226, RPMI-8226, and NCC-IT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH .)
PVR/CD155 recombinant antibody
Rabbit anti-PVR/CD155 Recombinant Monoclonal Antibody [BLR074G]
Gene Names
PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
PVR/CD155, Recombinant Antibody; Rabbit anti-PVR/CD155 Recombinant Monoclonal Antibody [BLR074G]; HVED; PVS; poliovirus receptor; TAGE4; nectin-like protein 5; CD155; CD antigen CD155; NECL5; Necl-5; Poliovirus receptor; PVR/CD155 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR074G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for PVR/CD155 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Residues 28-329 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human PVR/CD155 by western blot. Samples: Whole cell lysate (10 ug) from 786-O, K-562, OVCAR-4, Jurkat, KM12, NCI-H226, RPMI-8226, and NCC-IT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH .)
WB (Western Blot)
(Detection of human PVR/CD155 by western blot. Samples: Whole cell lysate (10 ug) from 786-O, K-562, OVCAR-4, Jurkat, KM12, NCI-H226, RPMI-8226, and NCC-IT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH .)
IHC (Immunohistochemistry)
(Detection of human PVR/CD155 in FFPE hepatic carcinoma by IHC-IF. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry)
(Detection of human PVR/CD155 in FFPE hepatic carcinoma by IHC-IF. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemisry)
(Detection of human PVR/CD155 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human PVR/CD155 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PVR/CD155 by immunocytochemistry. Sample: FFPE section of HT29 cells. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PVR/CD155 by immunocytochemistry. Sample: FFPE section of HT29 cells. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM/FACS (Flow Cytometry)
(Detection of human PVR/CD155 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human PVR/CD155 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PVR/CD155 recombinant monoclonal antibody (AAA213562) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for PVR/CD155 recombinant antibody
PVR/CD155 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It mediates NK cell adhesion and triggers NK cell effector functions. PVR/CD155 binds two different NK cell receptors: CD96 and CD226, and these interactions accumulate at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. PVR/Cd155 also promotes NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. PVR/CD155 also plays a role in mediating tumor cell invasion and migration. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P15151]."
Product Categories/Family for PVR/CD155 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,303 Da
NCBI Official Full Name
poliovirus receptor isoform alpha
NCBI Official Synonym Full Names
poliovirus receptor
NCBI Official Symbol
PVR
NCBI Official Synonym Symbols
PVS; HVED; CD155; NECL5; TAGE4; Necl-5
NCBI Protein Information
poliovirus receptor; nectin-like protein 5
UniProt Protein Name
Poliovirus receptor
UniProt Gene Name
PVR
UniProt Synonym Gene Names
PVS; NECL-5
UniProt Entry Name
PVR_HUMAN
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Product Notes
The PVR/CD155 pvr (Catalog #AAA213562) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PVR/CD155 Recombinant Monoclonal Antibody [BLR074G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PVR/CD155 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PVR/CD155 pvr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PVR/CD155, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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