Mouse CD169 Monoclonal Antibody | anti-CD169 antibody
MOUSE ANTI PIG CD169
Gene Names
SIGLEC-1; SA; SN; pSn; p210; SIGLEC1
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
CD169, Antibody; MOUSE ANTI PIG CD169; anti-CD169 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3B11/11
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1730
Applicable Applications for anti-CD169 antibody
WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
Histology Positive Control Tissue
Porcine spleen
Buffer Solution
Phosphate buffered saline
Immunogen
Porcine alveolar macrophages
Target Species
Pig
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Related Product Information for anti-CD169 antibody
Mouse anti Pig CD169, clone 3B11/11 Is specific for porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003). Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer's patches. Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011). Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
sialoadhesin
NCBI Official Symbol
SIGLEC-1
NCBI Official Synonym Symbols
SA; SN; pSn; p210; SIGLEC1
NCBI Protein Information
sialoadhesin; Sialic acid-binding Ig-like lectin 1
UniProt Protein Name
Sialoadhesin
UniProt Gene Name
SIGLEC1
UniProt Synonym Gene Names
SA; SN; pSn; Siglec-1
UniProt Entry Name
SN_PIG
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Product Notes
The CD169 siglec1 (Catalog #AAA49666) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD169 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD169 siglec1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD169, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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