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FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD171 antibody L1-14.10 (AAA72139). Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of L1-14.10 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Human CD171 Monoclonal Antibody | anti-CD171 antibody
Anti-CD171 [L1-14.10]
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunofluorescence
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD171, Antibody; Anti-CD171 [L1-14.10]; L1CAM; 14.10; L1; Neural cell adhesion molecule L1; N-CAM-L1; NCAM-L1; anti-CD171 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
L1-14.10
Specificity
This antibody is specific for the ectodomain (amino acids M1 to E1123) of human L1, and recognizes an epitope in the third Ig domain. L1 is over-expressed in a range of tumours, including neuroblastomas, renal carcinomas, ovarian and endometrial carcinomas, and melanomas. This antibody does not cross-react with human CHL1.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD171 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence)
Immunogen
This antibody was raised by immunizing mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
PMID
16867862
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD171 antibody L1-14.10 (AAA72139). Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of L1-14.10 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-CD171 antibody L1-14.10 (AAA72139). Paraformaldehyde fixed HeLa cells were stained with anti-unknown specificity antibody or the mouse IgG1 version of L1-14.10 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
WB (Western Blot)
(Western Blot using anti-CD171 antibody L1-14.10 (AAA72139). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD171 antibody L1-14.10 (AAA72139). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells using anti-CD171 (AAA72139) L1-14.10 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of L1-14.10 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells using anti-CD171 (AAA72139) L1-14.10 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric mouse IgG version of L1-14.10 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.)
WB (Western Blot)
(Western Blot using anti-CD171 antibody L1-14.10 (AAA72139). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
WB (Western Blot)
(Western Blot using anti-CD171 antibody L1-14.10 (AAA72139). HeLa(A) (0.03ug/ml), Kelly(B) cells (0.01ug/ml), human brain cerebellum(C) (0.003ug/ml) and cerebral cortex(D) (0.01ug/ml) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of L1-14.10 before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Related Product Information for anti-CD171 antibody
Recombinant monoclonal antibody to CD171. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma L1-14.10.
This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
This antibody was raised by immunising mice with recombinant L1-Fc fusion protein, consisting of the ectodomain of human L1.
Product Categories/Family for anti-CD171 antibody
References
Huszar et al. Expression profile analysis in multiple human tumors identifies L1 (CD171) as a molecular marker for differential diagnosis and targeted therapy. Hum Pathol. 2006 Aug;37(8):1000-8. Epub 2006 Jun 21.
Note on publication: Describes the original generation of this antibody, and its use in immunohistochemical analysis.
Note on publication: Describes the original generation of this antibody, and its use in immunohistochemical analysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neural cell adhesion molecule L1 isoform 1
NCBI Official Synonym Full Names
L1 cell adhesion molecule
NCBI Official Symbol
L1CAM
NCBI Official Synonym Symbols
S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
NCBI Protein Information
neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
UniProt Protein Name
Neural cell adhesion molecule L1
UniProt Gene Name
L1CAM
UniProt Synonym Gene Names
CAML1; MIC5; N-CAM-L1; NCAM-L1
UniProt Entry Name
L1CAM_HUMAN
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Product Notes
The CD171 l1cam (Catalog #AAA72139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD171 [L1-14.10] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD171 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot), IF (Immunofluorescence). Researchers should empirically determine the suitability of the CD171 l1cam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD171, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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