FCM/FACS (Flow Cytometry)
(Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 ug/mL,left) or CD271/NGFR Rabbit mAb(AAA283029,2 ug/mL,right).)
Rabbit anti-Mouse CD271 Monoclonal Antibody | anti-Ngfr antibody
CD271 Rabbit mAb
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Affinity purification
Synonyms
CD271, Antibody; CD271 Rabbit mAb; p75; LNGFR; p75NTR; p75NGFR; Tnfrsf16; CD271; anti-Ngfr antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
KETCSTGMYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECLGLQSMSAPCVEADDAVCRCSYGYYQDEETGRCEACSVCGVGSGLVFSCQDKQNTVCEECPEGTYSDEANHVDPCLPCTVCEDTERQLRECTPWADAECEEIPGRWITRSTPPEGSDVTTPSTQEPEAPPERDLIASTVADTVTTVMGSSQPVVTRGTADNL
Applicable Applications for anti-Ngfr antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Cross Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 32-254 of mouse CD271 (NP_150086.2).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 ug/mL,left) or CD271/NGFR Rabbit mAb(AAA283029,2 ug/mL,right).)
FCM/FACS (Flow Cytometry)
(Flow cytometry:1X10^6 293F(Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 ug/mL,left) or CD271/NGFR Rabbit mAb(AAA283029,2 ug/mL,right).)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293F cells (negative control,Left) and 293F(Transfection) cells (Right) were surface-stained with CD271/NGFR Rabbit mAb(AAA283029,2ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2ug/mL,blue line),followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 293F cells (negative control,Left) and 293F(Transfection) cells (Right) were surface-stained with CD271/NGFR Rabbit mAb(AAA283029,2ug/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2ug/mL,blue line),followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb(1:200 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).)
WB (Western Blot)
(Western blot analysis of lysates from Mouse ovary, using CD271 Rabbit mAb (AAA283029) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of lysates from Mouse ovary, using CD271 Rabbit mAb (AAA283029) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Related Product Information for anti-Ngfr antibody
Enables death receptor activity; nerve growth factor binding activity; and small GTPase binding activity. Involved in several processes, including cellular glucose homeostasis; circadian regulation of gene expression; and dorsal aorta development. Acts upstream of or within several processes, including hair follicle morphogenesis; nervous system development; and regulation of signal transduction. Located in several cellular components, including cell surface; cell-cell junction; and postsynaptic density. Is expressed in several structures, including alimentary system; genitourinary system; integumental system; nervous system; and sensory organ. Human ortholog(s) of this gene implicated in Alzheimer's disease and mental depression. Orthologous to human NGFR (nerve growth factor receptor).
Product Categories/Family for anti-Ngfr antibody
NCBI and Uniprot Product Information
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Product Notes
The Ngfr ngfr (Catalog #AAA283029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD271 Rabbit mAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD271 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the Ngfr ngfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KETCSTGMYT HSGECCKACN LGEGVAQPCG ANQTVCEPCL DSVTFSDVVS ATEPCKPCTE CLGLQSMSAP CVEADDAVCR CSYGYYQDEE TGRCEACSVC GVGSGLVFSC QDKQNTVCEE CPEGTYSDEA NHVDPCLPCT VCEDTERQLR ECTPWADAEC EEIPGRWITR STPPEGSDVT TPSTQEPEAP PERDLIASTV ADTVTTVMGS SQPVVTRGTA DNL. It is sometimes possible for the material contained within the vial of "CD271, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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