<
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(AAA290919). AAA290919 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Mouse anti-Human CD276 Monoclonal Antibody | anti-CD276 antibody
CD276 Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified through a protein G column, followed by dialysis against PBS.
Synonyms
CD276, Antibody; CD276 Antibody; CD276 antigen; 4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD276; B7H3; anti-CD276 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
2009CT652.35.9
Specificity
This CD276 antibody is generated from a mouse immunized with a recombinant protein from the human region of human CD276.
Purity/Purification
Purified through a protein G column, followed by dialysis against PBS.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
534
Applicable Applications for anti-CD276 antibody
ELISA, WB (Western Blot), IHC (Immunohistochemistry)
Antigen Source
Human
Cellular Location
Membrane; Single-pass type I membrane protein
Tissue Location
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(AAA290919). AAA290919 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human tonsil section using Pink1(AAA290919). AAA290919 was diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
WB (Western Blot)
(Dilution: WB - 1:4000All lanes: Anti-CD276 Antibody at 1:4000 dilution Lane 1: 293T-17 whole cell lysate Lane 2: A-431 whole cell lysate Lane 3: U-20S whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Hela whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB - 1:4000All lanes: Anti-CD276 Antibody at 1:4000 dilution Lane 1: 293T-17 whole cell lysate Lane 2: A-431 whole cell lysate Lane 3: U-20S whole cell lysate Lane 4: LNCap whole cell lysate Lane 5: Hela whole cell lysate Lane 6: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohiostchemistry)
(Dilution: IHC-P - 1:500Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Dilution: IHC-P - 1:500Immunohistochemical analysis of CD276 Antibody in 293T(untransfected) cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Dilution: IHC-P - 1:25Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Dilution: IHC-P - 1:25Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Related Product Information for anti-CD276 antibody
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Product Categories/Family for anti-CD276 antibody
References
Chapoval A.I.,et al.Nat. Immunol. 2:269-274(2001). Steinberger P.,et al.J. Immunol. 172:2352-2359(2004). Clark H.F.,et al.Genome Res. 13:2265-2270(2003). Ota T.,et al.Nat. Genet. 36:40-45(2004). Otsuki T.,et al.DNA Res. 12:117-126(2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Calculated MW: 57235
NCBI Official Full Name
B7-H3 protein
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
UniProt Entry Name
CD276_HUMAN
Similar Products
Product Notes
The CD276 cd276 (Catalog #AAA290919) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD276 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD276 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD276, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.