IF (Immunofluorescence)
(Immunofluorescence of CD276 in human spleen tissue with CD276 antibody at 20 μg/ml.Red: CD276 Antibody (8689)Blue: DAPI staining)
Rabbit CD276 Polyclonal Antibody | anti-CD276 antibody
CD276 Antibody
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
CD276, Antibody; CD276 Antibody; CD276 molecule; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; anti-CD276 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
At least three antibodies are known to exist; this antibody will detect all three isoforms.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
534
Applicable Applications for anti-CD276 antibody
WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
CD276 antibody was raised against a peptide corresponding to 19 amino acids near the carboxy terminus of CD276.
The immunogen is located within amino acids 484 - 534 of CD276.
The immunogen is located within amino acids 484 - 534 of CD276.
Buffer
CD276 Antibody is supplied in PBS containing 0.02% sodium azide.
Species
Human
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of CD276 in human spleen tissue with CD276 antibody at 20 μg/ml.Red: CD276 Antibody (8689)Blue: DAPI staining)
IF (Immunofluorescence)
(Immunofluorescence of CD276 in human spleen tissue with CD276 antibody at 20 μg/ml.Red: CD276 Antibody (8689)Blue: DAPI staining)
IHC (Immunohiostchemistry)
(Immunohistochemistry of CD276 in human spleen tissue with CD276 antibody at 2 μg/ml.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of CD276 in human spleen tissue with CD276 antibody at 2 μg/ml.)
WB (Western Blot)
(Western blot analysis of CD276 in (A) human spleen and (B) mouse spleen tissue lysate with CD276 antibody at 0.5 μg/mL.)
WB (Western Blot)
(Western blot analysis of CD276 in (A) human spleen and (B) mouse spleen tissue lysate with CD276 antibody at 0.5 μg/mL.)
Related Product Information for anti-CD276 antibody
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Product Categories/Family for anti-CD276 antibody
References
1. Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74. 2. Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7. 3. Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51. 4. Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe?. Int J Cancer 2014; 134:2764-71.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 35, 43, 57 kDa
Observed: 59 kDa
Observed: 59 kDa
NCBI Official Full Name
CD276 antigen isoform a
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
NCBI Protein Information
CD276 antigen
UniProt Protein Name
CD276 antigen
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
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Product Notes
The CD276 cd276 (Catalog #AAA40871) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD276 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD276 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CD276 cd276 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD276, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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