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WB (Western Blot)
(Detection of human CD30 by western blot. Samples: Whole cell lysate (50 ug) from MJ, HEK293T, SR, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
CD30 recombinant antibody
Rabbit anti-CD30 Recombinant Monoclonal Antibody [BLR055F]
Gene Names
TNFRSF8; CD30; Ki-1; D1S166E
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
CD30, Recombinant Antibody; Rabbit anti-CD30 Recombinant Monoclonal Antibody [BLR055F]; D1S166E; tumor necrosis factor receptor superfamily member 8; TNFRSF8; lymphocyte activation antigen CD30; Ki-1; cytokine receptor CD30; CD30L receptor; CD30; CD antigen CD30; Ki-1 antigen; Tumor necrosis factor receptor superfamily member 8; TNF receptor superfamily member 8; CD30 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR055F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for CD30 recombinant antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 19-385 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD30 by western blot. Samples: Whole cell lysate (50 ug) from MJ, HEK293T, SR, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human CD30 by western blot. Samples: Whole cell lysate (50 ug) from MJ, HEK293T, SR, GaMG, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human CD30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552 lot 2) used for IP at 20 ul/mg lysate. CD30 was also immunoprecipitated by a previous lot of this antibody (AAA213552 lot 1) and a second antibody against a different epitope of CD30 (BL16009). For blotting immunoprecipitated CD30, AAA213552 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human CD30 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552 lot 2) used for IP at 20 ul/mg lysate. CD30 was also immunoprecipitated by a previous lot of this antibody (AAA213552 lot 1) and a second antibody against a different epitope of CD30 (BL16009). For blotting immunoprecipitated CD30, AAA213552 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
ICC (Immunocytochemistry)
(Detection of human CD30 by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
ICC (Immunocytochemistry)
(Detection of human CD30 by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Rabbit anti-CD30 recombinant monoclonal antibody (AAA213552) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
FCM/FACS (Flow Cytometry)
(Detection of human CD30 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-CD30 recombinant monoclonal (AAA213552) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human CD30 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-CD30 recombinant monoclonal (AAA213552) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for CD30 recombinant antibody
CD30 is a member of the TNF-receptor superfamily, expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity [provided by RefSeq, Jul 2008] [taken from NCBI Entrez Gene (Gene ID: 943)].
Product Categories/Family for CD30 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,935 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 8 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 8
NCBI Official Symbol
TNFRSF8
NCBI Official Synonym Symbols
CD30; Ki-1; D1S166E
NCBI Protein Information
tumor necrosis factor receptor superfamily member 8
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 8
UniProt Gene Name
TNFRSF8
UniProt Synonym Gene Names
CD30; D1S166E
UniProt Entry Name
TNR8_HUMAN
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Product Notes
The CD30 tnfrsf8 (Catalog #AAA213552) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD30 Recombinant Monoclonal Antibody [BLR055F] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CD30 tnfrsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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