Mouse anti-Human, Dog CD34 Monoclonal Antibody | anti-CD34 antibody

CD34

Cat. Num. AAA59186

• Reactivity: Human, Dog
• Synonyms: CD34, Antibody; CD34; anti-CD34 antibody

• Host: Mouse
• Reactivity: Human, Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: QBEnd/10
• Form/Format: CD34 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Immunogen: Human endothelial vesicles.
• Control: Tonsil, Placenta, Appendix
• Localization: Cytoplasmic, Membranous
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. This product contains <0.1% sodium azide (NaN3) as a preservative. Ensure proper handling procedures are used with this reagent.; 2. Always wear personal protective equipment such as laboratory coat, goggles and gloves when handling reagents.; 3. Dispose of unused solution with copious amount of water.; 4. Do not ingest reagent. If reagent is ingested, seek medical advice immediately.; 5. Avoid contact with eyes. If contact occurs, flush with large quantities of water.; 6. Follow safety precautions of the heating device used for epitope retrieval (TintoRetriever Pressure Cooker or similar).; 7. For additional safety information refer to Safety Data Sheet for this product.; 8. For complete recommendations for handling biological specimens, please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (see References in this document).
• Mounting Protocols: For detailed instructions using biodegradable permanent mounting media such as XyGreen PermaMounter or organic solvent based resin such as PermaMounter, please inquire.
• Preparation and Storage: Store at 2-8°C (Control Slides: Store at 20-25°C); ; Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

IHC (Immunohistochemistry)

(IHC of CD34 on a FFPE Kidney Tissue)

Application Data

(ABBREVIATED IMMUNOHISTOCHEMICAL PROTOCOL)

Application Data

(PRODUCT LIMITATIONS)

Application Data

Related Product Information for anti-CD34 antibody

CD34 functions as a cell-cell adhesion factor and cell-surface glycoprotein. It may also mediate the attachment of stem cells to bone marrow extracellular matrixes or directly to stromal cells. Cells expressing CD34 are normally found in the umbilical cord and bone marrow as hematopoletic cells, and in vascular endothelium. In addition to stem cell recognition, CD34 is expressed by vascular endothelium; it appears that proliferating endothelial cells express this molecule in greater amounts than resting cells. In comparison to factor VIII R Antigen, CD34 stains are stronger and appear to be more sensitive in nature. In tumors, CD34 is found in Alveolar Soft Part Sarcoma, pre B-ALL (positive in 75%), AML(40%), AMLM7 (most), Dermatofibrosarcoma Protuberans, Gastrointestinal Stromal Tumors, Giant Cell Fibroblastoma, Granulocytic Sarcoma, Kaposi's Sarcoma, Liposarcoma, Malignant Fibrous Histiocytoma, Malignant Peripheral Nerve Sheath tumors, Mengingeal Hemangiopericytomas, Meningiomas, Neurofibromas, Schwannomas, and Papillary Thyroid Carcinoma. A negative CD34 may exclude Ewing's Sarcoma/PNET, Myofibrosarcoma of the breast, and Inflammatory Myofibroblastic tumors of the stomach.

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• UniProt Accession #: Q3C1E7; P28906; Q5JTA5; Q7Z375; Q8IUM0; Q9UH97
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34; CD34 antigen; OTTHUMP00000034733; OTTHUMP00000034734
• UniProt Protein Name: CD34 antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: Q3C1E7_HUMAN