Mouse anti-Human CD36 Monoclonal Antibody | anti-CD36 antibody

anti-human CD36-Biotin

Cat. Num. AAA78157

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Reactivity: Human
• Synonyms: CD36, Antibody; anti-human CD36-Biotin; Monoclonal anti-human CD36/Biotin; anti-CD36 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgMk
• Clone Number: SMO
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN₃ (as a preservative).
• Concentration: 1.0 mg/ml (varies by lot)

• Immunogen: Human tonsil cells/PBM
• Production: Antibody from (low FBS containing) tissue culture supernatant was size exclusion purified to 95% Immunoglobulin by SDS-PAGE, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Performance: Five x 10⁵ ficoll prepared human peripheral blood mononuclear cells (PBMC) were washed and pre incubated ~5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD36/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2^o reagent Streptavidin/R-PE (MBS666773), after which they were washed three times, fixed and analyzed by FACS. A 7.5% sub population of cells stained positive with a mean shift of 2.3 log₁₀ fluorescent units when compared to a Mouse IgM/Biotin negative control (MBS666594).
• Preparation and Storage: Store at 2 - 5°C. Do not freeze.

FCM/FACS (Flow Cytometry)

Related Product Information for anti-CD36 antibody

Human CD36 is a major platelet glycoprotein also found on monocytes, macrophages and some endothelial cells. CD36 may play a role in cell adhesion as a receptor for thrombospondin. CD36 is a receptor for adhesion of malaria infected erythrocytes. CD36 is also proported to have a role in phagocytosis. Antibody SMO recognizes the CD36 molecule of 88 kd.

References

N. Hogg, et al, (1984) Immunology 53: 753-767. P. Oquendo, et al, (1989) Cell 58: 95-101. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1269-1289. Y. Ren, et al, (1995) J Exp Med 181: 1857-1862.

NCBI and Uniprot Product Information

• NCBI GI #: 12620274
• NCBI GeneID: 948
• NCBI Official Full Name: CD36, partial
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4; GPIIIB; PAS IV; PAS-4 protein; glycoprotein IIIb; cluster determinant 36; fatty acid translocase; platelet glycoprotein IV; scavenger receptor class B, member 3; leukocyte differentiation antigen CD36; CD36 antigen (collagen type I receptor, thrombospondin receptor)
• UniProt Protein Name: Platelet glycoprotein 4
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV
• UniProt Entry Name: CD36_HUMAN