Mouse anti-Human CD37 Monoclonal Antibody | anti-CD37 antibody

CD37 (Peripheral Mature B-Cell Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62736

• Gene Names: CD37; GP52-40; TSPAN26
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD37, Antibody; CD37 (Peripheral Mature B-Cell Marker) Mouse Monoclonal Antibody; Cell differentiation antigen 37; GP52 40; Tetraspanin-26; Tspan-26; TSPAN26; anti-CD37 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: IPO-24
• Specificity: Recognizes a protein of 33-55kDa, identified as CD37 (Workshop V; Code CD37.7). CD37 is strongly expressed on normal and neoplastic mature (sIg+) B-lymphocytes. In B-cell ontogeny, CD37 appears after the pre-B-cell stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells.1 CD37 is also present, at low densities, on resting and activated T cells, neutrophils, monocytes, and some myelomonocytic leukemia cells. It is absent from platelets, erythrocytes. CD37 is a member of a family of tetraspan transmembrane proteins, including CD9, CD53, CD63, CD81, and CD82. It associates other tetraspan transmembrane proteins and MHC class II molecules to form a large complex at the surface of B cells and play a role in signal transduction. CD37 is a valuable and stable marker for peripheral mature B-cells and corresponding malignancies like B-cell chronic lymphocytic leukemia (B-CLL), hairy cell leukemia (HCL), and all types of B-cell non-Hodgkin's lymphoma (B-NHL).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 213

• Applicable Applications for anti-CD37 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay)
• Cellular Localization: Cell Surface
• Immunogen: Spleen cells of a patient with hairy cell leukemia
• Hu-Chromosome Location: 19q13.3
• Positive Control: Daudi, Raji, Namalva, Ramos cells and human lymphocytes. Human tonsil and lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CD37 Mouse Monoclonal Antibody (IPO24). Confirmation of Purity and Integrity of Antibody.)

FCM/FACS (Flow Cytometry)

(Flow Cytometric staining of PHA stimulated PBMC. Green isotype control; Red CD37 Mouse Monoclonal Antibody (IPO24). Goat anti-Mouse PE was used as secondary antibody)

References

Schlossman SF et al. eds. Leukocyte Typing V, p556-559, Oxford University Press, Oxford, 1995. Sidorenko SP et al. Monoclonal antibodies of the IPO series in studying and diagnosing malignant lymphoproliferative diseases. Gematol Transfuziol 1990, 35(4):19-22 Sidorenko SP et al. Monoclonal antibodies of IPO series against B cell differentiation antigens in leukemia and lymphoma immunophenotyping Neoplasma 1992;39(1):3-9. Maecker HT et al. The tetraspanin superfamily: molecular facilitators. FASEB J 1997,11(6):428-442 Angelisova P et al. Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 1994;39(4):249-256

NCBI and Uniprot Product Information

• NCBI GI #: 91807110
• NCBI GeneID: 951
• NCBI Accession #: NP_001035120.1
• NCBI GenBank Nucleotide #: NM_001040031.1
• UniProt Accession #: P11049; Q3KPF9; B4DVC1
• Molecular Weight: 33-55kDa
• NCBI Official Full Name: leukocyte antigen CD37 isoform B
• NCBI Official Synonym Full Names: CD37 molecule
• NCBI Official Symbol: CD37
• NCBI Official Synonym Symbols: GP52-40; TSPAN26
• NCBI Protein Information: leukocyte antigen CD37
• UniProt Protein Name: Leukocyte antigen CD37
• UniProt Gene Name: CD37
• UniProt Synonym Gene Names: TSPAN26; Tspan-26
• UniProt Entry Name: CD37_HUMAN